Cataloguenumber
CYT-1103
Synonyms
Angiopoietin-2,Angiopoietin2,ANGPT2,ANG2,ANG-2,ANGPT-2.
Introduction
ANGPT2akaAngiopoietin-2competesforbindingtotheTIE2receptorandblocksangiopoietin-1akaANGPT1inducedTIE2autophosphorylationduringvasculogenesis.ANGPT-2isanaturallyoccurringantagoNISTofANGPT-1.ANGPT2inducestyrosinephosphorylationofTEK/TIE2inthelackofAngiopoietin-2.InthedeficiencyofVEGFanangiogenicinducer,ANGPT-2inducesendothelialcellapoptosisresultinginvascularregression.ANGPT2alongwithVEGFenableendothelialcellmigrationandproliferation,resultinginpermissiveangiogenicsignal.
Description
ANGPT2producedinChinesehamsterovary(CHO)cellsbyrecombinantDNAtechnologyisasingle,polypeptidechain(19-496a.a.)andfusedtoa6aaHisTagatC-terminuscontainingatotalof484aminoacidsandhavingamolecularmassof55.7kDa.ANGPT2showsmultiplebandsbetween50-100kDaonSDS-PAGE,reducingconditionsandpurifiedbyproprietarychromatographictechniques.
Source
ChineseHamsterOvaryCells(CHO).
PhysicalAppearance
Sterilefilteredcolorlesssolution.
Formulation
ANGPT2proteinsolution(0.25mg/ml)containsPhosphatebufferedsaline(pH7.4)and10%glycerol.
StABIlity
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.Store,frozenat-20°Cforlongerperiodsoftime.Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).Avoidmultiplefreeze-thawcycles.
Purity
Greaterthan90.0%asdeterminedbySDS-PAGE.
Aminoacidsequence
YNNFRKSMDSIGKKQYQVQHGSCSYTFLLPEMDNCRSSSSPYVSNAVQRDAPLEYDDSVQRLQVLENIMENNTQWLMKLENYIQDNMKKEMVEIQQNAVQNQTAVMIEIGTNLLNQTAEQTRKLTDVEAQVLNQTTRLELQLLEHSLSTNKLEKQILDQTSEINKLQDKNSFLEKKVLAMEDKHIIQLQSIKEEKDQLQVLVSKQNSIIEELEKKIVTATVNNSVLQKQQHDLMETVNNLLTMMSTSNSAKDPTVAKEEQISFRDCAEVFKSGHTTNGIYTLTFPNSTEEIKAYCDMEAGGGGWTIIQRREDGSVDFQRTWKEYKVGFGNPSGEYWLGNEFVSQLTNQQRYVLKIHLKDWEGNEAYSLYEHFYLSSEELNYRIHLKGLTGTAGKISSISQPGNDFSTKDGDNDKCICKCSQMLTGGWWFDACGPSNLNGMYYPQRQNTNKFNGIKWYYWKGSGYSLKATTMMIRPADFHHHHHH
SafetyDataSheet
SDS
