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Prospec/TFB1M Human/Transcription Factor B1, Mitochondrial Human Recombinant/2µg/PRO-903-2µg-2µg
Cataloguenumber
PRO-903
Synonyms
Dimethyladenosinetransferase1mitochondrial,Mitochondrial12SrRNAdimethylase1,MitochondrialtranscriptionfactorB1,h-mtTFB,h-mtTFB1,hTFB1M,mtTFB1,S-adenosylmethionine-6-N"-N"-adenosyl(rRNA)dimethyltransferase1,TFB1M,CGI-75,CGI75,mtTFB.
Introduction
TFB1Misamemberofthemethyltransferasesuperfamily.TFB1Misadimethyltransferasewhichmethylatesmitochondrial12SrRNAattheconservedstemloop.TheTFB1Mproteinalsoispartofthebasalmitochondrialtranscriptioncomplexandisrequiredformitochondrialgeneexpression.TFB1Mstimulatestranscriptionindependentlyofthemethyltransferaseactivity.
Description
TFB1MHumanRecombinantproducedinE.Coliisasingle,non-glycosylatedpolypeptidechaincontaining340aminoacids(28-346a.a.)andhavingamolecularmassof38.8kDa.TFB1Misfusedtoa21aminoacidHis-tagatN-terminus&purifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Formulation
TFB1Mproteinsolution(0.5mg/ml)containing20mMTris-HClbuffer(pH8.0),20%glycerol,0.1MNaCland1mMDTT.
StABIlity
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.Store,frozenat-20°Cforlongerperiodsoftime.Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).Avoidmultiplefreeze-thawcycles.
Purity
Aminoacidsequence
MGSSHHHHHHSSGLVPRGSHMQAAKQLSQNFLLDLRLTDKIVRKAGNLTNAYVYEVGPGPGGITRSILNADVAELLVVEKDTRFIPGLQMLSDAAPGKLRIVHGDVLTFKVEKAFSESLKRPWEDDPPNVHIIGNLPFSVSTPLIIKWLENISCRDGPFVYGRTQMTLTFQKEVAERLAANTGSKQRSRLSVMAQYLCNVRHIFTIPGQAFVPKPEVDVGVVHFTPLIQPKIEQPFKLVEKVVQNVFQFRRKYCHRGLRMLFPEAQRLESTGRLLELADIDPTLRPRQLSISHFKSLCDVYRKMCDEDPQLFAYNFREELKRRKSKNEEKEEDDAENYRL.
SafetyDataSheet
SDS