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Prospec/HAO1 Mouse/Hydroxyacid Oxidase 1 Mouse Recombinant/100µg/ENZ-1104-100µg-100µg

Cataloguenumber
ENZ-1104
Synonyms
(S)-2-hydroxy-acidoxidase;EC1.1.3.15,Glycolateoxidase,GOX,GOX1MGC142227;GOXMGC142225,HAO1,HAO-1,HAOX1,hydroxyacidoxidase(glycolateoxidase)1,hydroxyacidoxidase1,HydroxyacidOxidase1,HydroxyacidOxidase-1.
Introduction
HydroxyacidOxidase1(HAO1)isapartofthesuperfamilyofthealphahydroxyacidoxidases(HAO)enzymes.HAO1catalysestheFMNmediatedoxidationofglycolatetoglyoxylateandglyoxylatetooxalatebyreducingoxygentohydrogenperoxide.HAO1isexpressedmainlyintheliverandpancreasandismostactiveontwocarbonsubstratessuchasglycolate.HAO1isthemaincauseofhyperoxaluria,adisorderinwhichlargedepositsofcalciumoxalateformkidneystones.
Description
HAO1MouseRecombinantproducedinE.coliisasingle,non-glycosylatedpolypeptidechaincontaining393aminoacids(1-370)andhavingamolecularmassof43.4kDa.HAO1isfusedtoa23aminoacidHis-TagatN-terminusandpurifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Formulation
HAO1protein(1mg/ml)iscontainingPhosphateBufferedSaline(pH7.4)and10%glycerol.
StABIlity
Purity
Greaterthan90.0%asdeterminedbySDS-PAGE.
BIOLOGicalActivity
Specificactivityis>1,000pmol/min/ug,anddefinedastheamountofenzymethatoxidizeglyoxylateatpH8.0at25C.
Aminoacidsequence
MGSSHHHHHHSSGLVPRGSHMGSMLPRLVCISDYEQHVRSVLQKSVYDYYRSGANDQETLADNIQAFSRWKLYPRMLRNVADIDLSTSVLGQRVSMPICVGATAMQCMAHVDGELATVRACQTMGTGMMLSSWATSSIEEVAEAGPEALRWMQLYIYKDREISRQIVKRAEKQGYKAIFVTVDTPYLGNRIDDVRNRFKLPPQLRMKNFETNDLAFSPKGNFGDNSGLAEYVAQAIDPSLSWDDITWLRRLTSLPIVVKGILRGDDAKEAVKHGVDGILVSNHGARQLDGVPATIDVLPEIVEAVEGKVEVFLDGGVRKGTDVLKALALGAKAVFVGRPIIWGLAFQGEKGVQDVLEILKEEFRLAMALSGCQNVKVIDKTLVRKNPLAVSKI.
SafetyDataSheet
SDS