Prospec/肠激酶-牛/肠肽酶/肠激酶-轻链-牛重组体/20IU/ENZ-311-20IU-20IU
商品编号：
ENZ-311-20IU-20IU
品牌：
prospecbio
市场价：
¥2000.00
美元价：
900.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Catalogue number
ENZ-311
Synonyms
Enteropeptidase, EC 3.4.21.9, Enterokinase, Serine protease 7, ENTK, MGC133046.
Introduction
Enteropeptidase or enterokinase is an enzymeinvolved in human digestion. It is produced by cells in the duodenum wall, and is secreted from duodenum"s glands, the crypts of Lieberk?hn, whenever ingested food enters the duodenum from the stomach. Enteropeptidase has the critical job of turning trypsinogen(a zymogen) to trypsin, indirectly activating a number of pancreaticdigestive enzymes.Enteropeptidase is a serine proteaseenzyme(EC3.4.21.9). Enteropeptidase is a part of the Chymotrypsin-clan of serine proteases, and is structurally similar to these proteins.
Description
Enterokinase (rEK) Bovine Recombinant is the catalytic subunit of bovine enterokinase, which is expressed by the yeast and purified to yield a high enzyme activity preparation. EK recognizes the sequence Asp-Asp-Asp-Asp-Lys and cleaves the peptide bond after the lysine residue. The enzyme can be used to cleave any fusion protein that carries this sequence.Recombinant Bovine Enterokinase is a single glycosylated polypeptide chain containing 235 amino acids and having an MW of ~43kDa.
Source
Pichia pastoris.
Physical Appearance
Sterile liquid solution.
Formulation
Bovine EK in 50mM Tris-HCl, pH 8.0, 0.5M NaCl and 50% glycerol.
Stability
One year when stored at –20°C. Please avoid freeze-thaw cycles.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Unit Definition
One unit is defined as the amount of enzyme needed to cleave 50µg of fusion protein in 16 hours to 95% completion at 25°C in a buffer containing 25mM Tris-HCl, pH 7.6, 50mM NaCl, and 2mM CaCl2.
Assay Conditions
Each reaction contains 50 µg of partially purified thioredoxin-NP-27 fusion protein and varying amounts of Bovine EK. Reactions were incubated at 25°C for 16 hours and analyzed on a Coomassiestained 15% SDS gel.
Safety Data Sheet
SDS
Usage
Prospec"s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
品牌介绍
ProSpec-Tany TechnoGene Ltd.公司创立人Dr. Tanchum (Tany) Amarant 主要致力于蛋白生物化学方面的研究工作，1947年出生于以色列。1991-1997创建ReProGen 公司，从事细胞因子和趋化因子的大量生产及纯化；1997-2000创建ProSpec-TechnoGene，主要生产用于生命科学研究的各种重组蛋白及相关抗体。拥有专利United States Patent 5434135。
ProSpec is an evolving biotech company providing highly purified proteins worldwide which serve the research community with cytokine-related products for cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cell research.
Among our proteins are Interferons, Interleukins, Bone Morphogenic Proteins, Tumor Necrosis factors, Leptins, Stem Cell factors, Prolactins, Chemokines, Antibodies, Peptides, Enzymes, Viral Antigens, various growth factors such as: Platelet Derived, Epidermal, Insulin Like, Nerve, Connective Tissue, Vascular Endothelial, Fibroblast and more.....
Our research team and scientists have been able to assemble more than 1700 recombinant proteins, peptides and antibodies for your research needs. The proteins are rigorously tested to meet the research and development demand for excellent quality, uncompromising biological activity at competitive prices.
ProSpec has established sterling reputation among the scientific community, a reputation built on innovative products, customer service and attention to detail. Our reputation for quality is an essential factor in achieving a favorable position in the market place and the benefits associated with manufacturing top quality products.
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