Cataloguenumber
CYT-1003
Synonyms
LeukemiaInhibitoryFactor,DifferentiationInhibitoryActivity,CholinergicDifferentiationFactor,Differentiation-StimulatingFactor,Hepatocyte-StimulatingFactorIII,Differentiation-InducingFactor,Melanoma-DerivedLPLInhibitor,HumanInterleukinInDACells,DFactor,HILDA,MLPLI,Emfilermin,DIA,CDF,Leukemiainhibitoryfactor,LIF,Differentiation-stimulatingfactor,Dfactor,Melanoma-derivedLPLinhibitor,MLPLI,Emfilermin.
Introduction
LeukemiaInhibitoryFactoralsocalledLIFisalymphoidfactorthatpromoteslong-termmaintenanceofembryonicstemcellsbysuppressingspontaneousdifferentiation.LeukemiaInhibitoryFactorhasseveralfunctionssuchascholinergicneurondifferentiation,controlofstemcellpluripotency,bone&fatmetabolism,mitogenesisoffactordependentcelllines&promotionofmegakaryocyteproductioninvivo.HumanandmouseLIFexhibita78%identityinitsaminoacidsequence.
Description
LIFHumanRecombinantproducedinSf9Baculoviruscellsisasingle,glycosylatedpolypeptidechaincontaining189aminoacids(23-202a.a.)andhavingamolecularmassof20.8kDa(MolecularsizeonSDS-PAGEwillappearatapproximately18-40kDa).LIFisexpressedwitha6aminoacidHistagatC-Terminusandpurifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Formulation
LIFproteinsolution(0.25mg/ml)containsPhosphateBufferedSaline(pH7.4)and10%glycerol.
StABIlity
Purity
Aminoacidsequence
BIOLOGicalActivity
MeasuredinacellproliferationassayusingTF-1humanerythroleukemiccell.TheED50forthiseffectsislessorequalto0.5ng/ml.
SafetyDataSheet
SDS
Usage
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.