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Prospec/VHL PAT82B10AT Antibody/Von Hippel-Lindau Protein, Clone PAT82B10AT, Mouse Anti Human/100µg/ANT-722-100µg-100µg
Cataloguenumber
ANT-722
Synonyms
VonHippel-Lindaudiseasetumorsuppressor,pVHL,ProteinG7,VHL,RCA1,VHL1,HRCA1.
Type
Introduction
VonHippel-Lindaudiseaseisadominantinheritedsyndromecharacterizedbythepredispositiontodevelopvariouskindsofbenignandmalignanttumors,includingclearcellrenalcarcinomas,pheochromocytomasandhemangioblastomasofthecentralnervoussystemandretina.VHLsyndromeiscausedbygermlinemutationintheVHLtumorsuppressor,andVHLtumorsareassociatedwithlossormutationoftheremainingwild-typeallele.VHLhastwodomains:aroughly100-residueNH2-terminaldomainrichinbsheet(b-domain)andasmallera-helicaldomain(a-domain),heldtogetherbytwolinkersandapolarinterface.VHLproteinisalsoinvolvedinthedegradationofhypoxia-inducIBLefactor(HIF).
Clone
PAT82B10AT.
Immunogen
Anti-humanVHLmAb,isderivedfromhybridizationofmouseF0myelomacellswithspleencellsfromBALB/cmiceimmunizedwitharecombinanthumanVHLprotein1-154aminoacidspurifiedfromE.coli.
IgSubclass
MouseIgG2bheavychainandklightchain.
PurificationMethod
VHLantibodywaspurifiedfrommouseasciticfluidsbyprotein-Gaffinitychromatography.
Formulation
1mg/mlcontainingPBS,pH-7.4,&0.1%SodiumAzide.
StorageProcedures
StABIlity/ShelfLife
Applications
VHLantibodyhasbeentestedbyELISAandWesternblotanalysistoassurespecificityandreactivity.Sinceapplicationvaries,however,eachinvestigationshouldbetitratedbythereagenttoobtainoptimalresults.
SafetyDataSheet
SDS