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Prospec/NME2 Antibody/Non-Metastatic Cells 2, Mouse Anti Human/20µg/ANT-706-20µg-20µg
Cataloguenumber
ANT-706
Synonyms
NucleosidediphosphatekinaseB,NDPK-B,NDPKB,NM23-H2,NM23B,EC2.7.4.6,NDPkinaseB,C-mycpurine-bindingtranscriptionfactorPUF,NDKB,NME2,puf,MGC111212.
Type
Introduction
NME2takesanimportantpartinthesynthesisofnucleosidetriphosphatesotherthanATP.NME2negativelycontrolsRhoactivitybyinteractingwithAKAP13/LBC.NME2actsasatranscriptionalactivatoroftheMYCgene.NME2bindsDNAnon-specifically.NME2isaheterodimericenzymefunctioningasanucleosidediphosphatekinase.NME1andNME2contain152aminoacids,AandBpolypeptidechainsoftheNM23enzyme,respectively.NME2isidenticaltothebetasubunitofhumanerythrocyteNDPkinase.NDPkinasesparticipateinthesynthesisofnucleosidetriphosphates,andNM23isinvolvedintheregulationofsignaltransductionbycomplexingwithGproteins,causingactivation/inactivationofdevelopmentalpathways.
Clone
PAT5F4AT.
PhysicalAppearance
Immunogen
Anti-humanNME2mAb,isderivedfromhybridizationofmouseF0myelomacellswithspleencellsfromBALB/cmiceimmunizedwithrecombinanthumanNME2aminoacids1-152purifiedfromE.coli.
IgSubclass
MouseIgG2bheavychainandκlightchain.
PurificationMethod
NME2antibodywaspurifiedfrommouseasciticfluidsbyprotein-Aaffinitychromatography.
Formulation
1mg/mlcontainingPBS,pH-7.4,10%Glyceroland0.02%SodiumAzide.
StorageProcedures
StABIlity/ShelfLife
Applications
NME2antibodyhasbeentestedbyELISAandWesternblotanalysistoassurespecificityandreactivity.Sinceapplicationvaries,however,eachinvestigationshouldbetitratedbythereagenttoobtainoptimalresults.
SafetyDataSheet
SDS