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Prospec/BPGM Antibody/2,3-Bisphosphoglycerate Mutase, Mouse Anti Human/20µg/ANT-685-20µg-20µg
Cataloguenumber
ANT-685
Synonyms
Bisphosphoglyceratemutase,EC5.4.2.4,BPGM,2,3-bisphosphoglyceratemutaseerythrocyte,2,3-bisphosphoglyceratesynthase,BPG-dependentPGAM.
Type
Introduction
BPGMisfoundathighconcentrationsinredbloodcellswhereitbindstoanddecreasestheoxygenaffinityofhemoglobin.PGMdeficiencyincreasestheoxygenaffinityofcells.BPGMisamultifunctionalenzymethatcatalyzes2,3-DPGsynthesisthroughitssynthetaseactivity,and2,3-DPGdegradationusingitsphosphataseactivity.BPGMhasphosphoglyceratephosphomutaseactivity.MutationsinBPGMcausehemolyticanemia.BPGMcatalyzesthereactionofEC5.4.2.1(mutase)andEC3.1.3.13(phosphatase),butwithareducedactivity.
Clone
PA2E11AT.
PhysicalAppearance
Immunogen
Anti-humanBPGMmAb,isderivedfromhybridizationofmouseF0myelomacellswithspleencellsfromBALB/cmiceimmunizedwithrecombinanthumanBPGMaminoacids1-259purifiedfromE.coli.
IgSubclass
PurificationMethod
BPGMantibodywaspurifiedfrommouseasciticfluidsbyprotein-Aaffinitychromatography.
Formulation
1mg/mlcontainingPBS,pH-7.4,10%Glyceroland0.02%SodiumAzide.
StorageProcedures
StABIlity/ShelfLife
Applications
BPGMantibodyhasbeentestedbyELISA,Westernblotanalysis,FlowcytometryandICC/IFtoassurespecificityandreactivity.Sinceapplicationvaries,however,eachinvestigationshouldbetitratedbythereagenttoobtainoptimalresults.
SafetyDataSheet
SDS