Cataloguenumber
PRO-551
Synonyms
Introduction
Alpha-2Macroglobulinisaserineproteasesinhibitor,whichinhibitscoagulationbyinactivatingthrombinandKallikrein,itinhibitsfibrinolysisbyinactivatingplasminandinvolvedintransport.Alpha-2-Macroglobulinisalargeplasmaprotein,whichisproducedbytheliver,it’scomposedof4identicalsubunitsboundtogetherby-S-S-bonds.A2Misabletoinactivatemanykindsofproteinases(includingserine-,cysteine-,aspartic-andmetalloproteinases).A2Mhasa35aminoacid"bait"regioninitsstructure.Proteinasesbindandcleavethe“bait”regionbecomeboundtoA2M.Macrophagereceptorsrecognizetheproteinase-A2Mcomplexandclearitfromthesystem.A2MbindstoandremovesMMP-2andMMP-9(activeformsofthegelatinase)fromthecirculationusingscavengerreceptorsonthephagocytes.ThelevelsofAlpha-2-macroglobulinareincreasedinnephroticsyndromewhichisaconditionwherethekidneysstarttoleakoutsomeofthesmallerbloodproteins.Duetoitslargesize,A2-macroglobulinisretainedinthebloodstream.IncreasedproductionofallproteinscausesA2-macroglobulinconcentrationtoincrease.Chronicrenalfailuremightleadtoamyloidbyalpha-2-macroglobulin.A2Misraisedincirrhosis,pregnancyanddiabetes.
Description
Source
PhysicalAppearance
Formulation
Lyophilizedfromaconcentratedsolutioncontaining5mMpotassiumphosphatebuffer,pH6.5and1:1ratio(w/w)ofGlycine.
Solubility
StABIlity
LyophilizedA2Mproteinalthoughstableatroomtemperaturefor3weeks,shouldbestoreddesiccatedbelow-18°C.UponreconstitutionandfiltersterilizationA2Mcanbestoredat4°Cforupto4weeks.Forlongtermstorageandmoredilutedsolutionsitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Pleasepreventfreeze-thawcycles.
HumanVirusTest
Purity
Greaterthan95.0%.
SafetyDataSheet
SDS