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主营:主营:重组和天然蛋白、细胞因子和生长因子、趋化因子、蛋白激酶、激素和酶、热休克蛋白、病毒抗原、单克隆及多克隆抗体。
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当前位置: 首页 > 产品中心 > Other_natural_proteins > Prospec/Thrombin Bovine/Bovine Thrombin/100000U/PRO-447-100000U-100000U
商品详细Prospec/Thrombin Bovine/Bovine Thrombin/100000U/PRO-447-100000U-100000U
Prospec/Thrombin Bovine/Bovine Thrombin/100000U/PRO-447-100000U-100000U
Prospec/Thrombin Bovine/Bovine Thrombin/100000U/PRO-447-100000U-100000U
商品编号: PRO-447-100000U-100000U
品牌: prospecbio
市场价: ¥10000.00
美元价: 4250.00
产地: 美国(厂家直采)
公司:
产品分类: 其它天然蛋白
公司分类: Other_natural_proteins
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

Catalogue number

PRO-447

Synonyms

Coagulation Factor II (Thrombin), Prepro-Coagulation Factor II, EC 3.4.21.5, RPRGL2, THPH1, Coagulation Factor II, Prothrombin B-Chain, Serine Protease, Prothrombin, EC 3.4.21, PT, F2.

Introduction

Thrombin enzyme (Activated Factor IIa) is an important clotting promoter that controls the transformation of soluble fibrinogen to insoluble active fibrin strands. Thrombin is a coagulation protein and a serine protease (EC 3.4.21.5) that catalyzes many coagulation-related reactions. Thrombin triggers factor-XI, factor-V, Factor-XIII and factor-VIII. Thrombin endorses platelet activation, using activation of protease-activated receptors on the platelet. As a result of its high proteolytic specificity, thrombin has become an important biochemical protein. The thrombin cleavage site (Leu-Val-Pro-Arg-Gly-Ser) is widely used in linker regions of recombinant fusion protein constructs. After the purification of the fusion protein, thrombin is used to cleave between the Arginine and Glycine residues of the cleavage site, efficiently removing the purification tag from the protein of interest with a high degree of specificity.

Source

Bovine Blood.

Physical Appearance

Sterile Filtered light brownlyophilized (freeze-dried) powder.

Formulation

Lyophilized freeze dry powder formulated with Tris buffer, HCl and sodium chloride, PH 7.5.

Solubility

It is recommended to reconstitute the lyophilized Thrombin in sterile 18MΩ-cm H2O.

Biological Activity

Activity was determined by the direct comparison to NIH Thrombin Reference Standard which uses 200ul of diluted plasma 1:1 with saline as a substrate and 100ul of albumin solution based on a modification of the method of Biggs. The clotting durations for determining thrombin activity are in the range of 15–25 seconds at 37C. The specific activity found was 176 NIH units per mg.

Stability

Lyophilized Thrombin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitutionThrmbin should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Safety Data Sheet

SDS

Usage

ProSpec"s products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
品牌介绍
    ProSpec-Tany TechnoGene Ltd.公司创立人Dr. Tanchum (Tany) Amarant 主要致力于蛋白生物化学方面的研究工作,1947年出生于以色列。1991-1997创建ReProGen 公司,从事细胞因子和趋化因子的大量生产及纯化;1997-2000创建ProSpec-TechnoGene,主要生产用于生命科学研究的各种重组蛋白及相关抗体。拥有专利United States Patent 5434135。       ProSpec is an evolving biotech company providing highly purified proteins worldwide which serve the research community with cytokine-related products for cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cell research.       Among our proteins are Interferons, Interleukins, Bone Morphogenic Proteins, Tumor Necrosis factors, Leptins, Stem Cell factors, Prolactins, Chemokines, Antibodies, Peptides, Enzymes, Viral Antigens, various growth factors such as: Platelet Derived, Epidermal, Insulin Like, Nerve, Connective Tissue, Vascular Endothelial, Fibroblast and more.....       Our research team and scientists have been able to assemble more than 1700 recombinant proteins, peptides and antibodies for your research needs. The proteins are rigorously tested to meet the research and development demand for excellent quality, uncompromising biological activity at competitive prices.       ProSpec has established sterling reputation among the scientific community, a reputation built on innovative products, customer service and attention to detail. Our reputation for quality is an essential factor in achieving a favorable position in the market place and the benefits associated with manufacturing top quality products.