Cataloguenumber
PRO-447
Synonyms
CoagulationFactorII(Thrombin),Prepro-CoagulationFactorII,EC3.4.21.5,RPRGL2,THPH1,CoagulationFactorII,ProthrombinB-Chain,SerineProtease,Prothrombin,EC3.4.21,PT,F2.
Introduction
Source
PhysicalAppearance
SterileFilteredlightbrownlyophilized(freeze-dried)powder.
Formulation
LyophilizedfreezedrypowderformulatedwithTrisbuffer,HClandsodiumchloride,PH7.5.
Solubility
BIOLOGicalActivity
ActivitywasdeterminedbythedirectcomparisontoNIHThrombinReferenceStandardwhichuses200ulofdilutedplasma1:1withsalineasasubstrateand100ulofalbuminsolutionbasedonamodificationofthemethodofBiggs.Theclottingdurationsfordeterminingthrombinactivityareintherangeof15–25secondsat37C.Thespecificactivityfoundwas176NIHunitspermg.
StABIlity
LyophilizedThrombinalthoughstableatroomtemperaturefor3weeks,shouldbestoreddesiccatedbelow-18°C.UponreconstitutionThrmbinshouldbestoredat4°Cbetween2-7daysandforfutureusebelow-18°C.Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).Pleasepreventfreeze-thawcycles.
SafetyDataSheet
SDS