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Prospec/Y.Enterocolitica (O:9) YopB/Yersinia Enterocolitica (O:9) YopB Recombinant/50µg/PRO-2575-50µg-50µg

Cataloguenumber
PRO-2575
Synonyms
ProteinYopD,pYV0054.
Introduction
GastroenteritiswhichhasbeenidentifiedascausedbyYersiniaEnterocoliticahasbeendiscoveredbyvirulentisolatesofthebacteria.Duringinfection,poresarebeingformedinthepresenceofadapterproteinYersiniosis.YopB"sInteractionwiththetargetmembranetoformporesenablestheentranceofYopEeffectorstothecytoplasmicspaceofthehost’scell.
Description
RecombinantYersiniaEnterocolitica(O:9)YopBproducedinSf9Baculoviruscellsisasingle,glycosylatedpolypeptidechainhavingacalculatedmolecularmassof43kDa.
Y.Enterocolitica(O:9)YopBisexpressedwitha-10xHistagatN-terminusandpurifiedbyproprietarychromatographictechniques.
Source
Sf9,Baculoviruscells.
PhysicalAppearance
SterileFilteredclearsolution.
Formulation
Y.Enterocolitica(O:9)YopBissuppliedin20mMHEPESbufferpH-7.6,250mMNaCland20%glycerol.
StABIlity
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.
Store,frozenat-20°Cforlongerperiodsoftime.
Avoidmultiplefreeze-thawcycles.
Purity
Greaterthan80.0%asdeterminedbySDS-PAGE.
ImmunologicalActivity
SafetyDataSheet
SDS