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Prospec/Trypsin-2 Human/Trypsin-2 Human Recombinant/1mg/PRO-770-1mg-1mg
Catalogue number
PRO-770
Introduction
Description
Recombinant Human Trypsin-2 expressed in E.Coli having an Mw of 24kDa is purified by standard chromatography techniques.
Source
Physical Appearance
Formulation
The protein was lyophilized without any additives.
Solubility
It is recommended to reconstitute the lyophilized Human Trypsin in sterile 1mM HCl or 50mM HAC not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability
Recombinant Human Trypsin although stable at room temp for 1 week, should be stored desiccated below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Purity
Unit Definition
One USP unit of trypsin activity will produce a Delta A253 of 0.003 per minute in a reaction volume of 3.0ml at pH7.6 and 25°C, with BAEE as a substrate (1cm light path).
Biological Activity
2,800 units/mg.
Applications
Trypsin digestion: the suggested ratio is 1:50 to 1:1000 (w/w).
Safety Data Sheet
SDS