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Prospec/Trypsin Porcine/Trypsin Porcine Recombinant/50mg/PRO-787-50mg-50mg
Cataloguenumber
PRO-787
Introduction
Description
RecombinantPorcineTrypsinisexpressedinE.coliandpurifiedbystandardchromatographytechniques.
Source
E.coli.
PhysicalAppearance
SterileFilteredlyophilizedpowder.
Formulation
ThePorcineTrypsinwaslyophilizedwithmannitolaspreservative.
Solubility
ItisrecommendedtoreconstitutethelyophilizedPorcineTrypsininsterile1mMHClor50mMHACnotlessthan100µg/ml,whichcanthenbefurtherdilutedtootheraqueoussolutions.
StABIlity
RecombinantPorcineTrypsinalthoughstableatroomtempfor1week,shouldbestoreddesiccatedbelow-18°C.
Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Pleasepreventfreeze-thawcycles.
BIOLOGicalActivity
4500USPunits/mgprotein.
UnitDefinition
OneUSPunitoftrypsinactivitywillproduceaDeltaA253of0.003perminuteinareactionvolumeof3.0mlatpH7.6and25°C,withBAEEasasubstrate(1cmlightpath).
Applications
Trypsindigestion:thesuggestedratiois1:50to1:1000(w/w).
Aminoacidsequence
VGGYTCAANSIPYQVSLNSGSHFCGGSLINSQWVVSAAHCYKSRIQVRLGEHNI
DVLEGNEQFINAAKIITHPNFNGNTLDNDIMLIKLSSPATLNSRVATVSLPRSCA
AAGTECLISGWGNTKSSGSSYPSLLQCLKAPVLSDSSCKSSYPGQITGNMICVGF
LEGGKDSCQGDSGGPVVCNGQLQGIVSWGYGCAQKNKPGVYTKVCNYVNWI
QQTIAAN
SafetyDataSheet
SDS