Catalogue number
PRO-787
Introduction
Description
Recombinant Porcine Trypsin is expressed in E.coli and purified by standard chromatography techniques.
Source
E.coli.
Physical Appearance
Sterile Filtered lyophilized powder.
Formulation
The Porcine Trypsin was lyophilized with mannitol as preservative.
Solubility
It is recommended to reconstitute the lyophilized Porcine Trypsin in sterile 1mM HCl or 50mM HAC not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability
Recombinant Porcine Trypsin although stable at room temp for 1 week, should be stored desiccated below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Biological Activity
4500 USP units/mg protein.
Unit Definition
One USP unit of trypsin activity will produce a Delta A253 of 0.003 per minute in a reaction volume of 3.0ml at pH7.6 and 25°C, with BAEE as a substrate (1cm light path).
Applications
Trypsin digestion: the suggested ratio is 1:50 to 1:1000 (w/w).
Amino acid sequence
VGGYTCAANSIPYQVSLNSGSHFCGGSLINSQWVVSAAHCYKSRIQVRLGEHNI
DVLEGNEQFINAAKIITHPNFNGNTLDNDIMLIKLSSPATLNSRVATVSLPRSCA
AAGTECLISGWGNTKSSGSSYPSLLQCLKAPVLSDSSCKSSYPGQITGNMICVGF
LEGGKDSCQGDSGGPVVCNGQLQGIVSWGYGCAQKNKPGVYTKVCNYVNWI
QQTIAAN
Safety Data Sheet
SDS
