Cataloguenumber
PRO-691
Synonyms
JV18,MADH2,MADR2,JV18-1,hMAD-2,hSMAD2,MGC22139,MGC34440,SMAD2,Mothersagainstdecapentaplegichomolog2,MothersagainstDPPhomolog2,Mad-relatedprotein2,SMAD2,SMADfamilymember2.
Introduction
SMAD2ispartoftheSMADfamilyofproteinsthatmediatesignaltransductionbytheTGF-beta/BMP-2/4cytokinesuperfamilyfromreceptorSer/Thrproteinkinasesatthecellsurfacetothenucleus.PhosphorylatedSMAD2isthenabletoformacomplexwithSMAD4orSARA.Thesecomplexesaccumulateinthecellnucleus,wheretheyaredirectlyparticipatingintheregulationofgeneexpression.SMAD2mediatesthesignaloftheTGF-beta,andthereforeregulatesmultiplecellularprocesses,suchascellproliferation,apoptosis,anddifferentiation.SMAD2isrecruitedtotheTGF-betareceptorsthroughitsinteractionwiththeSMADanchorforreceptoractivation(SARA)protein.TGF-beta1actsonadjacentstromalcellstoturnonSMAD2signallingthatcouldleadtostromaldecidualization.SMAD2isthedownstreamsignaltransducersofTGF-beta-1inhumandentalpulpcells.
Description
SMAD2HumanRecombinantproducedinE.Coliisasingle,non-glycosylatedpolypeptidechaincontaining487aminoacids(1-467)andhavingamolecularmassof54.4kDa.SMAD2isfusedto20aminoacidHis-TagatN-Terminusandpurifiedbystandardchromatographytechniques.
Source
EscherichiaColi.
PhysicalAppearance
SterileFilteredcolorlesssolution.
Formulation
TheSMAD2proteinsolutioncontains20mMTris-HClpH-8,and100mMNaCl.
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.Store,frozenat-20°Cforlongerperiodsoftime.Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).Avoidmultiplefreeze-thawcycles.
Aminoacidsequence
MGSSHHHHHHSSGLVPRGSHMSSILPFTPPVVKRLLGWKKSAGGSGGAGGGEQNGQEEKWCEKAVKSLVKKLKKTGRLDELEKAITTQNCNTKCVTIPSTCSEIWGLSTPNTIDQWDTTGLYSFSEQTRSLDGRLQVSHRKGLPHVIYCRLWRWPDLHSHHELKAIENCEYAFNLKKDEVCVNPYHYQRVETPVLPPVLVPRHTEILTELPPLDDYTHSIPENTNFPAGIEPQSNYIPETPPPGYISEDGETSDQQLNQSMDTGSPAELSPTTLSPVNHSLDLQPVTYSEPAFWCSIAYYELNQRVGETFHASQPSLTVDGFTDPSNSERFCLGLLSNVNRNATVEMTRRHIGRGVRLYYIGGEVFAECLSDSAIFVQSPNCNQRYGWHPATVCKIPPGCNLKIFNNQEFAALLAQSVNQGFEAVYQLTRMCTIRMSFVKGWGAEYRRQTVTSTPCWIELHLNGPLQWLDKVLTQMGSPSVRCSSMS.
SafetyDataSheet
Purity
Greaterthan95.0%asdeterminedbySDS-PAGE.
Usage
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.