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Prospec/RSPO1 Human/R-Spondin-1 Human Recombinant/100µg/PRO-2593-100µg-100µg

Cataloguenumber
PRO-2593
Synonyms
R-spondin-1,Cristin-3,mCristin-3,Roofplate-specificspondin-1,Rspo1,RSPO,R-spondin1,R-spondin,Rspondin,CRISTIN3.
Introduction
R-Spondin-1(Rspo1)isapartoftheRspondinfamily.Rspo1playsaroleasanactivatorofthecanonicalWntsignalingpathwaybyactingasaligandforLGR4-6receptors.Rspo1inducestheonsetofcryptcellproliferationandincreasesintestinalepithelialhealingeffect.Rspo1isnegativelyregulatingtheTGF-betapathway.
Description
R-Spondin-1HumanRecombinantproducedinCHOcellsisaglycosylatedmonomerchaincontaining233aminoacidsandhavingatotalmolecularmassof25.6kDa.
RSPO1ispurifiedbyproprietarychromatographictechniques.
Source
CHOcells.
PhysicalAppearance
Formulation
Theproteinwaslyophilizedfromasterile(0.2µm)filteredsolutioncontainingPBS.
Solubility
ItisrecommendedtoreconstitutethelyophilizedRSPO1insterile18MΩ-cmH2Onotlessthan100µg/ml,whichcanthenbefurtherdilutedtootheraqueoussolutions.
StABIlity
LyophilizedRSPO1althoughstableatroomtemperaturefor3weeks,shouldbestoreddesiccatedbelow-18°C.UponreconstitutionRSPO1shouldbestoredat4°Cbetween2-7daysandforfutureusebelow-18°C.
Pleasepreventfreeze-thawcycles.
Aminoacidsequence
RISAEGSQACAKGCELCSEVNGCLKCSPKLFILLERNDIRQVGVCLPSCPPGYFDARNPDMNKCIKCKIEHCEACFSHNFCTKCKEGLYLHKGRCYPACPEGSSAANGTMECSSPAQCEMSEWSPWGPCSKKQQLCGFRRGSEERTRRVLHAPVGDHAACSDTKETRRCTVRRVPCPEGQKRRKGGQGRRENANRNLARKESKEAGAGSRRRKGQQQQQQQGTVGPLTSAGPA.
Purity
Greaterthan95.0%asdeterminedbySDS-PAGE.
BIOLOGicalActivity
TheED50,ascalculatedbytheLuciferaseinductioninHEK-293STFcellsinthepresenceofMurineWnt-3ais24.7ng/mlcorrespondingtoaspecificactivityof4.0x10^4units/mg.
SafetyDataSheet
SDS