Prospec/MutS/Thermus Aquaticus DNA Mismatch Repair Protein MutS Recombinant/10µg/PRO-2573-10µg-10µg-免疫在线蚂蚁淘旗下平台

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商品详细Prospec/MutS/Thermus Aquaticus DNA Mismatch Repair Protein MutS Recombinant/10µg/PRO-2573-10µg-10µg

Prospec/MutS/Thermus Aquaticus DNA Mismatch Repair Protein MutS Recombinant/10µg/PRO-2573-10µg-10µg

商品编号： PRO-2573-10µg-10µg

品牌： prospecbio

市场价： ¥1000.00

美元价： 450.00

产地：美国(厂家直采)

公司：

产品分类：其它重组蛋白

公司分类： Other_recombinant_protein

联系Q Q： 3392242852

电话号码： 4000-520-616

电子邮箱： info@ebiomall.com

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商品介绍

Cataloguenumber

PRO-2573

Synonyms

MutS,ThermusAquaticusDNAMismatchRepairProtein,DNAmismatchrepairproteinMutS.

Introduction

TheMutSDNAMismatchProteinIdentifiesheteroduplexDNAscomprisingmis-pairedorunpairedbases.TheMutSDNAMismatchProteinbindsinvitrotoheteroduplexDNAscomprisingmis-pairedorunpairedbasesoveravariedtemperaturerangefrom4-70 °CandobtainsaThermostableATPaseactivity.TheMutSDNAMismatchProteinisactiveattemperaturebetween0to75°C.SinceTheMutSDNAMismatchProteincompetentlybindsto1-4basesdeletion(orinsertion)andmismatchbasepairsofGT,CTandAG,itissuitableforsensingthesemutations.MutationscanbedistinguishedinpolyacrylamidegelsoronasolidphasesuchasNiagaroseorbeadsormagneticNi-NTAparticles.

Description

DNAMismatchRepairProteinMutSThermusAquaticusRecombinantproducedinE.coliisasingle,non-glycosylatedpolypeptidechaincontaining829aminoacidsandhavingamolecularmassof92.8kDa.TheThermusAquaticusisfusedtoa6aminoacidHis-TagatC-terminusandpurifiedbyproprietarychromatographictechniques.

Source

EscherichiaColi.

PhysicalAppearance

Sterilefilteredcolorlesssolution.

Formulation

TheMutSproteinissuppliedin20mMTris-HCl,pH-8,250mMNaCl,0.1mMEDTA,1mMDTTand50%Glycerol.

StABIlity

Storeat4°Cifentirevialwillbeusedwithin2-4weeks.Store,frozenat-20°Cforlongerperiodsoftime.Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).Avoidmultiplefreeze-thawcycles.

ReactionConditions

100mMKCl,50mMTris-HCl,pH-8.5,20mMMgCl₂,0.1mMEDTA,1mMDTTand2%Glycerol,65°C.

Purity

Greaterthan95.0%asdeterminedbySDS-PAGE.

Aminoacidsequence

MKIEHMEGMLKGEGPGPLPPLLQQYVELRDQYPDYLLLFQVGDFYECFGEDAERLARALGLVLTHKTSKDFTTPMAGIPLRAFEAYAERLLKMGFRLAVADQVEPAEEAEGLVRREVTQLLTPGTLLQESLLPREANYLAAIATGDGWGLAFLDVSTGEFKGTVLKSKSALYDELFRHRPAEVLLAPELLENGAFLDEFRKRFPVMLSEAPFEPEGEGPLALRRARGALLAYAQRTQGGALSLQPFRFYDPGAFMRLPEATLRALEVFEPLRGQDTLFSVLDETRTAPGRRLLQSWLRHPLLDRGPLEARLDRVEGFVREGALREGVRRLLYRLADLERLATRLELGRASPKDLGALRRSLQILPELRALLGEEVGLPDLSPLKEELEAALVEDPPLKVSEGGLIREGYDPDLDALRAAHREGVAYFLELEERERERTGIPTLKVGYNAVFGYYLEVTRPYYERVPKEYRPVQTLKDRQRYTLPEMKEKEREVYRLEALIRRREEEVFLEVRERAKRQAEALREAARILAELDVYAALAEVAVRYGYVRPRFGDRLQIRAGRHPVVERRTEFVPNDLEMAHELVLITGPNMAGKSTFLRQTALIALLAQVGSFVPAEEAHLPLFDGIYTRIGASDDLAGGKSTFMVEMEEVALILKEATENSLVLLDEVGRGTSSLDGVAIATAVAEALHERRAYTLFATHYFELTALGLPRLKNLHVAAREEAGGLVFYHQVLPGPASKSYGVEVAAMAGLPKEVVARARALLQAMAARREGALDAVLERLLALDPDRLTPLEALRLLQELKALALGAPLDTMKGKLAAALEHHHHHH.

Preparationprotocol

1.AfterfirstroundPCR,purifyPCRfragmentsusingQiagenQIAquickPCRpurificationkitwithelutionindH₂O.

2.DilutePCRproductto250ng/µlin10mMTris–HCl,pH7.8,50mMNaClandheatto95°Cfor5minfollowedbycoolingat0.1°C/sto25°C.

3.Addbindingbuffer(20mMTris–HCl,pH7.8,10mMNaCl,5mMMgCl₂,1mMDTTand5%glycerol)toannealedPCRproduct,adjustDNAconcentrationto11.5ng/µl,addMutSdimersto950nM.

4.Incubatethemixtureatroomtemperaturefor10min,thenaddanequalvolumeofNi-NTAbeadspre-equilibratedwith1×bindingbuffer,andincubatefor30minatroomtemperature.

5.Gentlyspindownbeadsandsavesupernatantforsubsequentprocessing(secondroundPCR,cloningetc).

Applications

RecommendedApplications:

1.RemovemismatchDNA(errorcorrection)fromgenesynthesisreaction

2.Mutationdetectionandremoval

3.RapidisothermalSNPdetection

SafetyDataSheet

SDS

Usage

Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.

品牌介绍

ProSpec-Tany TechnoGene Ltd.公司创立人Dr. Tanchum (Tany) Amarant 主要致力于蛋白生物化学方面的研究工作，1947年出生于以色列。1991-1997创建ReProGen 公司，从事细胞因子和趋化因子的大量生产及纯化；1997-2000创建ProSpec-TechnoGene，主要生产用于生命科学研究的各种重组蛋白及相关抗体。拥有专利United States Patent 5434135。 ProSpec is an evolving biotech company providing highly purified proteins worldwide which serve the research community with cytokine-related products for cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cell research. Among our proteins are Interferons, Interleukins, Bone Morphogenic Proteins, Tumor Necrosis factors, Leptins, Stem Cell factors, Prolactins, Chemokines, Antibodies, Peptides, Enzymes, Viral Antigens, various growth factors such as: Platelet Derived, Epidermal, Insulin Like, Nerve, Connective Tissue, Vascular Endothelial, Fibroblast and more..... Our research team and scientists have been able to assemble more than 1700 recombinant proteins, peptides and antibodies for your research needs. The proteins are rigorously tested to meet the research and development demand for excellent quality, uncompromising biological activity at competitive prices. ProSpec has established sterling reputation among the scientific community, a reputation built on innovative products, customer service and attention to detail. Our reputation for quality is an essential factor in achieving a favorable position in the market place and the benefits associated with manufacturing top quality products.

公司介绍

品牌分类

细胞因子天然蛋白质类重组蛋白单克隆抗体神经营养素激素多克隆抗体 CD抗原趋化因子

酶病毒抗原生长因子

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