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Other_recombinant_protein >
Prospec/SOS1 Human/Son Of Sevenless Homolog 1 Human Recombinant/50µg/PRO-2507-50µg-50µg
Cataloguenumber
PRO-2507
Synonyms
SOSRas/RacGuanineNucleotideExchangeFactor1,GingivalFibromatosis,Hereditary,1,SOS-1,SonOfSevenlessHomolog1(Drosophila),GuanineNucleotideExchangeFactor,SonOfSevenlessHomolog1,GINGF,GGF1,GF1,HGF,NS4,Sonofsevenlesshomolog1.
Introduction
SOS1,alsoknownasGingivalFibromatosis,isaRas&Racguaninenucleotideexchangefactor.SOS1iscomprisedofanumberofsignificantdomains.TheREMandCDC25domainsprovidethecatalyticactivityofSOS1towardsRasandthehistonefoldDH/PH(Dblhomology&Pleckstrinhomology)domainsfunction,intandem,tostimulateGTP/GDPexchangeforRac.Moreover,bindingofGTPactivatesRasproteins,andsubsequenthydrolysisoftheboundGTPtoGDPandphosphateinactivatessignalingbytheseproteins.GTPbindingcanbecatalyzedbyguaninenucleotideexchangefactorsforRAS,andGTPhydrolysiscanbeenhancedbyGTPase-activatingproteins.
Description
SOS1producedinSf9Baculoviruscellsisasingle,glycosylatedpolypeptidechaincontaining495aminoacids(564-1049a.a.)andhavingamolecularmassof58.0kDa.(MolecularsizeonSDS-PAGEwillappearatapproximately50-70kDa).SOS1isexpressedwitha6aminoacidHistagatC-Terminusandpurifiedbyproprietarychromatographictechniques.
Source
Sf9,Baculoviruscells.
PhysicalAppearance
Formulation
SOS1proteinsolution(0.25mg/ml)contains20mMTris-HCl(pH7.5),30%glycerol,0.1MNaCl,1mMDTT&0.2mMMgCl2.
StABIlity
Purity
Aminoacidsequence
ADPEEQMRLPSADVYRFAEPDSEENIIFEENMQPKAGIPIIKAGTVIKLIERLTYHMYADPNFVRTFLTTYRSFCKPQELLSLIIERFEIPEPEPTEADRIAIENGDQPLSAELKRFRKEYIQPVQLRVLNVCRHWVEHHFYDFERDAYLLQRMEEFIGTVRGKAMKKWVESITKIIQRKKIARDNGPGHNITFQSSPPTVEWHISRPGHIETFDLLTLHPIEIARQLTLLESDLYRAVQPSELVGSVWTKEDKEINSPNLLKMIRHTTNLTLWFEKCIVETENLEERVAVVSRIIEILQVFQELNNFNGVLEVVSAMNSSPVYRLDHTFEQIPSRQKKILEEAHELSEDHYKKYLAKLRSINPPCVPFFGIYLTNILKTEEGNPEVLKRHGKELINFSKRRKVAEITGEIQQYQNQPYCLRVESDIKRFFENLNPMGNSMEKEFTDYLFNKSLEIEPRNPKPLPRFPKKYSYPLKSPGVRPSNPRPGTHHHHHH.
SafetyDataSheet
SDS