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Prospec/ctxB/Cholera Toxin B subunit Recombinant/50µg/PRO-2605-50µg-50µg
Cataloguenumber
PRO-2605
Synonyms
CholeraenterotoxinsubunitB,CholeraenterotoxinBchain,Choleraenterotoxingammachain,Choleragenoid,ctxB,toxB.
Introduction
CholeraToxinBsubunit(ctxB)CholeraisaproteincomplexsecretedbythebacteriumVibriocholerae.ctxBisresponsIBLeforthemassive,waterydiarrheacharacteristicofcholerainfection.Thecholeratoxinisanoligomericcomplexmadeupof6proteinsubunits:asinglecopyoftheAsubunitand5copiesoftheBsubunit,denotedasAB5.SubunitBbindswhilesubunitAactivatestheGproteinwhichactivatesadenylatecyclase.ThefiveBsubunitsformafive-memberedring.TheAsubunithas2importantsegments.TheA1portionofthechain(CTA1)isaglobularenzymepayloadthatADP-ribosylatesGproteins,whiletheA2chain(CTA2)formsanextendedalphahelixwhichsitssnuglyinthecentralporeoftheBsubunitring.
Description
CholeraToxinBsubunitRecombinantproducedinE.Coliisasingle,non-glycosylatedpolypeptidechaincontaining103aminoacidsandhavingamolecularmassof11.6kDa.ctxBispurifiedbyproprietarychromatographictechniques.
Source
EscherichiaColi.
PhysicalAppearance
Sterilefilteredcolorlesssolution.
Formulation
ctxBissuppliedasa0.2μmfilteredsolutionconteining5mMPB,pH7.0,75mMNaCl,and50%glycerol.
StABIlity
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.Store,frozenat-20°Cforlongerperiodsoftime.Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).Avoidmultiplefreeze-thawcycles.
Purity
Greaterthan98.0%asdeterminedby:(a)AnalysisbyRP-HPLC.(b)AnalysisbySDS-PAGE.
Aminoacidsequence
TPQNITDLCAEYHNTQIYTLNDKIFSYTESLAGKREMAIITFKNGAIFQVEVPGSQHIDSQKKAIERMKDTLRIAYLTEAKVEKLCVWNNKTPHAIAAISMAN.
SafetyDataSheet
SDS