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Prospec/CSTF1 Human, sf9/Cleavage Stimulation Factor 1 Human Recombinant, sf9/1mg/PRO-2436-1mg-1mg
Cataloguenumber
PRO-2436
Synonyms
CleavageStimulationFactorSubunit1,CleavageStimulationFactor,3Pre-RNA,Subunit1,50kD,CleavageStimulationFactor50KDaSubunit,CF-150KDaSubunit,CSTF50KDaSubunit,CstF-50,CleavageStimulationFactor,3Pre-RNA,Subunit1,50kDa,CleavageStimulationFactor,3Pre-RNA,Subunit1,CstFp50.
Introduction
Cleavagestimulationfactorsubunit1(CSTF1)isinvolvedinthepolyadenylationand3"endcleavageofpre-mRNAs.TheCSTF1geneencodesoneofthreesubunitswhichmergetoproducecleavagestimulationfactor(CSTF).LikethemammalianGproteinbetasubunits,CSTF1containstransducin-likerepeats.CSTF1isoneofthenumerousfactorsnecessaryforpolyadenylationand3"-endcleavageofmammalianpre-mRNAs.CSTF1isresponsIBLefortheinteractionofCSTFwithotherfactorstocreateastablecomplexonthepre-mRNA.
Description
CSTF1HumanRecombinantproducedinSf9Baculoviruscellsisasingle,glycosylatedpolypeptidechaincontaining437aminoacids(1-431a.a.)andhavingamolecularmassof49.1kDa(MolecularsizeonSDS-PAGEwillappearatapproximately40-57kDa).CSTF1isexpressedwitha6aminoacidsHistagatC-Terminusandpurifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Formulation
CSTF1proteinsolution(0.5mg/ml)containsPhosphateBufferedSaline(pH7.4),1mMDTTand20%glycerol.
StABIlity
Purity
Greaterthan90.0%asdeterminedbySDS-PAGE.
Aminoacidsequence
MYRTKVGLKDRQQLYKLIISQLLYDGYISIANGLINEIKPQSVCAPSEQLLHLIKLGMENDDTAVQYAIGRSDTVAPGTGIDLEFDADVQTMSPEASEYETCYVTSHKGPCRVATYSRDGQLIATGSADASIKILDTERMLAKSAMPIEVMMNETAQQNMENHPVIRTLYDHVDEVTCLAFHPTEQILASGSRDYTLKLFDYSKPSAKRAFKYIQEAEMLRSISFHPSGDFILVGTQHPTLRLYDINTFQCFVSCNPQDQHTDAICSVNYNSSANMYVTGSKDGCIKLWDGVSNRCITTFEKAHDGAEVCSAIFSKNSKYILSSGKDSVAKLWEISTGRTLVRYTGAGLSGRQVHRTQAVFNHTEDYVLLPDERTISLCCWDSRTAERRNLLSLGHNNIVRCIVHSPTNPGFMTCSDDFRARFWYRRSTTDHHHHHH.
SafetyDataSheet
SDS