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Prospec/BTLA Human/B And T Lymphocyte Associated Human Recombinant/1mg/PRO-2505-1mg-1mg

Cataloguenumber
PRO-2505
Synonyms
B-andT-lymphocyteattenuator,B-andT-lymphocyte-associatedprotein,CD272,BAndTLymphocyteAssociated,B-AndT-Lymphocyte-AssociatedProtein,B-AndT,LymphocyteAttenuator,CD272Antigen,BTLA1.
Introduction
BTLA,alsoknownasBAndTLymphocyteAssociated,isaninhibitorymoleculewhichispartoftheIgsuperfamily.BTLAisatype1transmembraneglycoproteinintheCD28familyofTcellcostimulatorymolecules.BTLAisa3rdinhibitoryreceptoronTlymphocyteswithresemblancestoCTLA-4&PD-1.Moreover,BTLAisaligandforTNF(receptor)superfamily,TNFRSF14,andHVEM.BTLA-HVEMcomplexesnegativelyregulateT-cellimmuneresponses.
Description
BTLAproducedinSf9Insectcellsisasingle,glycosylatedpolypeptidechaincontaining136aminoacids(31-157a.a.)andhavingamolecularmassof15.7kDa(MolecularsizeonSDS-PAGEwillappearatapproximately18-28kDa).BTLAisexpressedwitha9aminoacidHistagatC-Terminusandpurifiedbyproprietarychromatographictechniques.
Source
Sf9,Insectcells.
PhysicalAppearance
Sterilefilteredcolorlesssolution.
Formulation
BTLAproteinsolution(0.5mg/ml)containsPhosphateBufferedSaline(pH7.4)and10%glycerol.
StABIlity
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.Store,frozenat-20°Cforlongerperiodsoftime.Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).Avoidmultiplefreeze-thawcycles.
Purity
Greaterthan90.0%asdeterminedbySDS-PAGE.
Aminoacidsequence
ADPKESCDVQLYIKRQSEHSILAGDPFELECPVKYCANRPHVTWCKLNGTTCVKLEDRQTSWKEEKNISFFILHFEPVLPNDNGSYRCSANFQSNLIESHSTTLYVTDVKSASERPSKDEMASRPWLLYSHHHHHH.
SafetyDataSheet
SDS