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Prospec/LBP Human, HEK/Lipopolysaccarid Binding Protein, Human Recombinant, HEK/10µg/PRO-2581-10µg-10µg

Cataloguenumber
PRO-2581
Synonyms
Lipopolysaccharide-bindingprotein,LBP,MGC22233,Ly88.
Introduction
Lipopolysaccharides(LPS)areatypeofglycolipidsontheoutercellwallofGram-negativebacteria.Lipopolysaccharidebindingprotein(akaLBP)isaplasmaproteinwhichfacilitatesthediffusionofbacterialLPS(endotoxin).LBPisinvolvedintheacute-phaseimmunologicresponsetogram-negativebacterialinfections.IncooperationwithbactericidalpermeABIlity-increasingprotein(BPI),LBPbindsLPSandinteractswiththeCD14receptor,mostlikelyplayingaroleinregulatingLPS-dependentmonocyteresponses.LBPbelongstoafamilyofstructurallyandfunctionallyrelatedproteins,includingBPI,plasmacholesterylestertransferprotein(CETP),andphospholipidtransferprotein(PLTP).TheLBPgeneisfoundonchromosome20,directlydownstreamoftheBPIgene.LBPcatalyzesthetransferofLPSmonomersfromLPSaggregatestoHDLparticles,tophospholipidbilayers,andtoabindingsiteonsolubleCD14(sCD14).sCD14iscapableofspeedingupthetransferbyreceivinganLPSmonomerfromanLPSaggregate,andthenyieldingittoanHDLparticle,thereforeactingasasoluble"shuttle"foraninsolublelipid.
Description
LBPHumanRecombinantisasingle,glycosylatedpolypeptidechaincontaining462aminoacids(26-481a.a)andhavingamolecularmassof51.7kDa(calculated).LBPisfusedtoa6a.aHistagatC-terminal.
Source
HEK293Cells.
PhysicalAppearance
Formulation
LBPfiltered(0.4µm)andlyophilizedfrom0.5mg/mlsolutioninPBS,pH7.5and5%(w/v)Threalose.
Solubility
Itisrecommendedtoadddeionizedwatertoprepareaworkingstocksolutionofapproximately0.5mg/mlandletthelyophilizedpelletdissolvecompletely.
Stability
Purity
Greaterthan95.0%asdeterminedbySDS-PAGE.
Aminoacidsequence
ANPGLVARITDKGLQYAAQEGLLALQSELLRITLPDFTGDLRIPHVGRGRYEFHSLNIHSCELLHSALRPVPGQGLSLSISDSSIRVQGRWKVRKSFFKLQGSFDVSVKGISISVNLLLGSESSGRPTVTASSCSSDIADVEVDMSGDLGWLLNLFHNQIESKFQKVLESRICEMIQKSVSSDLQPYLQTLPVTTEIDSFADIDYSLVEAPRATAQMLEVMFKGEIFHRNHRSPVTLLAAVMSLPEEHNKMVYFAISDYVFNTASLVYHEEGYLNFSITDDMIPPDSNIRLTTKSFRPFVPRLARLYPNMNLELQGSVPSAPLLNFSPGNLSVDPYMEIDAFVLLPSSSKEPVFRLSVATNVSATLTFNTSKITGFLKPGKVKVELKESKVGLFNAELLEALLNYYILNTFYPKFNDKLAEGFPLPLLKRVQLYDLGLQIHKDFLFLGANVQYMRVHHHHHH.
SafetyDataSheet
SDS
Usage
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theymaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.