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Prospec/AGO2 (1-200) Human/Argonaute 2 (1-200 a.a.) Human Recombinant/10µg/PRO-2578-10µg-10µg
Cataloguenumber
PRO-2578
Synonyms
Proteinargonaute-2,Argonaute2,hAgo2,ArgonauteRISCcatalyticcomponent2,Eukaryotictranslationinitiationfactor2C2,eIF-2C2,eIF2C2,PAZPiwidomainprotein,PPD,AGO2,EIF2C2,Proteinslicer.
Introduction
TheArgonauteproteinispartoftheRISCorRNA-inducedsilencingcomplex,asso,theproteinhasakeypartintheslicingprocessesofRNA.TheRNAinterference(RNAi)isbeingheldbyRISC.Smallnon-codingRNAfragmentsbondtotheArgonauteproteins,throughbasepairing,eventuallyleadstothecleavageofmessengerRNAortranslationsuppression.
Description
AGO2(1-200)HumanRecombinantisasingle,non-glycosylated,polypeptidechaincontaining210aminoacids(1-200a.a)andhavingamolecularmassof23.7kDa(calculated).AGO2(1-200)isfusedtoa10a.aHistagatN-terminal.
Source
PhysicalAppearance
Formulation
AGO2(1-200)filtered(0.4µm)andlyophilizedfrom0.5mg/mlin50mMacetatebuffer,pH4.
Solubility
Itisrecommendedtoadd0.1Macetatebuffer,pH4toprepareaworkingstocksolutionofapproximately0.5mg/mlandletthelyophilizedpelletdissolvecompletely.AGO2(1-200)isnotsterile!Pleasefiltertheproductbyanappropriatesterilefilterbeforeusingitinthecellculture.
StABIlity
Purity
Greaterthan90.0%asdeterminedbySDS-PAGE.
Aminoacidsequence
MKHHHHHHASMYSGAGPALAPPAPPPPIQGYAFKPPPRPDFGTSGRTIKLQANFFEMDIPKIDIYHYELDIKPEKCPRRVNREIVEHMVQHFKTQIFGDRKPVFDGRKNLYTAMPLPIGRDKVELEVTLPGEGKDRIFKVSIKWVSCVSLQALHDALSGRLPSVPFETIQALDVVMRHLPSMRYTPVGRSFFTASEGCSNPLGGGREVW.
SafetyDataSheet
SDS
Usage
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theymaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.