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Prospec/CST3 Mouse, Active/Cystatin-C Mouse Recombinant, Active/100µg/PRO-2433-100µg-100µg

Cataloguenumber
PRO-2433
Synonyms
Cystatin-C,Cystatin-3,CST3,CST3
Introduction
Cystatinsareasuperfamilyofcysteineproteinaseinhibitorsfoundinbothplantsandanimals.Theycompriseagroupofproteinaseinhibitors,widelydistributedintissuesandbodyfluids,andformtightcomplexeswithcysteineproteasessuchascathepsinB,H,LandS.CystatinC,asecretedmoleculeofthisfamily,isofinterestfrombiochemical,medicineandevolutionarypointsofview.CystatinC,withmolecularweightof13260Da,iscomposedof120aminoacids,lackscarbohydrateandhastwodisulfidebridgeslocatednearthecarboxylterminus.CystatinCisincreasedinpatientswithmalignantdiseases,andisrelatedtotheinsufficiencyofrenalfunctionandappearstobeabetterMarkerthancreatinine.Ontheotherhand,lowlevelsofcystatinCinvolvecausethebreakdownoftheelasticlaminaeand,subsequently,theatherosclerosisandaBDominalaorticaneurysm.
Description
CST3MouseproducedinSf9Insectcellsisasingle,glycosylatedpolypeptidechaincontaining126aminoacids(21-140a.a.)andhavingamolecularmassof14.2kDa(MolecularsizeonSDS-PAGEwillappearatapproximately13.5-18kDa).CST3isexpressedwitha6aminoacidHistagatC-Terminusandpurifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Formulation
CST3proteinsolution(1mg/ml)containsPhosphateBufferedSaline(pH7.4)and10%glycerol.
StABIlity
Purity
Aminoacidsequence
ATPKQGPRMLGAPEEADANEEGVRRALDFAVSEYNKGSNDAYHSRAIQVVRARKQLVAGVNYFLDVEMGRTTCTKSQTNLTDCPFHDQPHLMRKALCSFQIYSVPWKGTHSLTKFSCKNAHHHHHH
BIOLOGicalActivity
TheIC50valueis<1.0nM.TheinhibitoryfunctionofCystatin3onproteaseactivityofpapainwasmeasuredbyafluorometricassayusingZ-FR-AMCatpH7.5at25°C.
SafetyDataSheet
SDS
Usage
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.