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Prospec/C1QTNF5 Human/Complement C1q Tumor Necrosis Factor-Related Protein 5 Human Recombinant/10µg/PRO-2599-10µg-10µg

Cataloguenumber
PRO-2599
Synonyms
Ctrp5,MFRP.
Introduction
ComplementC1qTumorNecrosisFactor-RelatedProtein5(C1QTNF5)encodesashort-chaincollagenwhichisexpressedmainlyinsub-retinalpigmentepithelium,ciliaryepitheliumandADIposetissue.C1QTNF5isincreasedinmtDNA-depletedmyocytesandstimulatesthephosphorylationofAMPactivatedproteinkinase.C1QTNF5takespartintheadhesionoftheretinalpigmentepithelium(RPE)totheBruchMembrane.MutationsinC1QTNF5havebeenassociatedwithlate-onsetretinaldegeneration.
Description
C1QTNF5HumanproducedinE.Coliisasingle,non-glycosylatedpolypeptidechaincontaining253aminoacids(16-243.a.a)andhavingamolecularmassof26.4kDa.C1QTNF5Humanisfusedtoa25aminoacidHis-tagatN-terminus&purifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Formulation
TheC1QTNF5proteinsolution(0.25mg/ml)isformulatedin20mMTris-HClbuffer(pH8.5)containing30%glyceroland0.2MNaCl.
Aminoacidsequence
MGSSHHHHHHSSGLVPRGSHMGSHMSPPLDDNKIPSLCPGHPGLPGTPGHHGSQGLPGRDGRDGRDGAPGAPGEKGEGGRPGLPGPRGDPGPRGEAGPAGPTGPAGECSVPPRSAFSAKRSESRVPPPSDAPLPFDRVLVNEQGHYDAVTGKFTCQVPGVYYFAVHATVYRASLQFDLVKNGESIASFFQFFGGWPKPASLSGGAMVRLEPEDQVWVQVGVGDYIGIYASIKTDSTFSGFLVYSDWHSSPVFA.
Purity
Greaterthan85.0%asdeterminedbySDS-PAGE.
StABIlity
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.Store,frozenat-20°Cforlongerperiodsoftime.Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).Avoidmultiplefreeze-thawcycles.
SafetyDataSheet
SDS