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Prospec/COL4A3 Human, Biotin/Collagen Type IV Alpha 3 Human Recombinant, Biotinylated/100µg/PRO-2564-100µg-100µg
Prospec/COL4A3 Human, Biotin/Collagen Type IV Alpha 3 Human Recombinant, Biotinylated/100µg/PRO-2564-100µg-100µg
商品编号：
PRO-2564-100µg-100µg
品牌：
prospecbio
市场价：
¥18000.00
美元价：
7650.00
产品分类：
其它重组蛋白
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Catalogue number
PRO-2564
Synonyms
Collagen alpha-3(IV) chain, Goodpasture antigen, COL4A3, Glomerular Basal Membrane, GBM.
Introduction
Type IV collagen is a major structural component of basement membranes. It is a multimeric protein composed of 3 alpha subunits, which are encoded by 6 different genes, alpha 1 through alpha 6. Each of these alpha subunits can form a triple helix structure with 2 other subunits to form type IV collagen. The Goodpasture syndrome is a condition in which autoantibodies bind to the collagen molecules in the basement membranes of alveoli and glomeruli. The epitopes that elicit these autoantibodies are restricted basically to the non-collagenous C-terminal domain of the protein. There are numerous alternative transcripts that appear to be unique to the human COL4A3 and alternative splicing is limited to the six exons that encode this C-terminal domain. COL4A3 is also linked to an autosomal recessive form of Alport syndrome. The mutations contributing to the Alport syndrome are also situated within the exons that encode this C-terminal region. COL4A3 is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter. Several exons of COL4A3 are interspersed with exons of an uncharacterized gene which is on the opposite strand.
Description
Human α3 chain of collagen IV; identical with the antigen called "glomerular basal membrane antigen" (GBM). Calculated Molecular weight: 48kDa. Calculated isoelectric point: pH 8.9. cDNA coding for a minicollagen version of the human collagen IV α3 chain fused to a 6-histidine purification tag. The term minicollagen designates the removal of most of the epitope-less triplehelical collagenous region (situated between the N-terminal 7S domain and the C-terminal noncollagenous NC1 domain), which is a requirement for recombinant production of this antigen.
Source
Sf9 insect cells.
Physical Appearance
Sterile Filtered clear solution.
Formulation
COL4A3 Human biotin contains 20mM HEPES, pH 7.5, 0.02% SDS and 0.01mM EDTA.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
Avoid multiple freeze-thaw cycles.
Purity
Greater than 95% as determined by Capillary Electrophoresis.
Immunological functions
1.Binds IgG type human auto antibodies.2.Functional Streptavidin based ELISA test (analysis of positive/negative samples.)
Note
It has been reported that the immunodominant epitope of COL4A3 is a cryptic epitope that is not easily accessible to the corresponding autoantibodies. It is necessary to treat the protein under nonreducing conditions with a denaturant such as urea to unmask the epitopes (see Hellmark et al. in Autoantibodies, Peter, J.B. and Shoenfeld, Y., eds., Elsevier B.V., 1996, pp 291-298).
Safety Data Sheet
SDS
Usage
ProSpec"s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
品牌介绍
ProSpec-Tany TechnoGene Ltd.公司创立人Dr. Tanchum (Tany) Amarant 主要致力于蛋白生物化学方面的研究工作，1947年出生于以色列。1991-1997创建ReProGen 公司，从事细胞因子和趋化因子的大量生产及纯化；1997-2000创建ProSpec-TechnoGene，主要生产用于生命科学研究的各种重组蛋白及相关抗体。拥有专利United States Patent 5434135。
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ProSpec has established sterling reputation among the scientific community, a reputation built on innovative products, customer service and attention to detail. Our reputation for quality is an essential factor in achieving a favorable position in the market place and the benefits associated with manufacturing top quality products.
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