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Prospec/CHGA Human, Sf9/Chromogranin A Human Recombinant, Sf9/5µg/PRO-2513-5µg-5µg

Cataloguenumber
PRO-2513
Synonyms
CHGA,CGA,Chromogranin-A,VasostatinI,SP-I,PituitarysecretoryproteinI.
Introduction
Chromogranin-AIsoform1PreproproteinorCHGAispartoftheneuroendocrinesecretoryproteinsofthechromogranin/secretograninfamily.CHGAisaprecursorofnumerusenzymessuchaspancreastatin,catestatin,vasostatin-1,vasostatin-2,andparastatin.Theproteinactsasanegativeregulatortheneuroendocrineactivityofautocrineornearbycells(paracrine).CHGAcausesfurtherproductionofsecretorygranulesthatcontainsinsulininpancreaticisletbetacells.
Description
CHGAproducedinSf9Baculoviruscellsisasingle,glycosylatedpolypeptidechain(19-457a.a.)andfusedtoa6aaHisTagatC-terminuscontainingatotalof448aminoacidsandhavingamolecularmassof50kDa.CHGAshowsmultiplebandsbetween50-70kDaonSDS-PAGE,reducingconditionsandpurifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Formulation
CHGAproteinsolution(0.25mg/ml)containsPhosphateBufferedSaline(pH7.4),20%glycerol&0.1mMPMSF.
StABIlity
Purity
Greaterthan85.0%asdeterminedbySDS-PAGE.
Aminoacidsequence
ADLLPVNSPMNKGDTEVMKCIVEVISDTLSKPSPMPVSQECFETLRGDERILSILRHQNLLKELQDLALQGAKERAHQQKKHSGFEDELSEVLENQSSQAELKEAVEEPSSKDVMEKREDSKEAEKSGEATDGARPQALPEPMQESKAEGNNQAPGEEEEEEEEATNTHPPASLPSQKYPGPQAEGDSEGLSQGLVDREKGLSAEPGWQAKREEEEEEEEEAEAGEEAVPEEEGPTVVLNPHPSLGYKEIRKGESRSEALAVDGAGKPGAEEAQDPEGKGEQEHSQQKEEEEEMAVVPQGLFRGGKSGELEQEEERLSKEWEDSKRWSKMDQLAKELTAEKRLEGQEEEEDNRDSSMKLSFRARAYGFRGPGPQLRRGWRPSSREDSLEAGLPLQVRGYPEEKKEEEGSANRRPEDQELESLSAIEAELEKVAHQLQALRRGHHHHHH.
SafetyDataSheet
SDS