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Prospec/ARL14 Human/ADP-Ribosylation Factor-Like 14 Human Recombinant/1mg/PRO-936-1mg-1mg
Cataloguenumber
PRO-936
Synonyms
ADP-ribosylationfactor-likeprotein14,ADP-ribosylationfactor7,ARL14,ARF7,FLJ22595.
Introduction
ADP-ribosylationfactor-like14(ARL14)isadevelopmentallyregulatedmemberoftheADP-ribosylationfactor/ARF-likeprotein(ARF/ARL)familyofRas-relatedGTPases.ARL14isGTPasewhichrecruitsMYO1EtoMHCclassII-containingvesiclesviatheeffectorproteinC11orf46/ARF7EPandthuscontrolsthemovementofthesevesiclesalongtheactinCytoskeletonindendriticcells.MHC-IItransportwascontrolledbytheGTPaseARL14/ARF7,whichrecruitsthemotormyosin1EviaaneffectorproteinARF7EP.
Description
ARL14HumanRecombinantproducedinE.Coliisasingle,non-glycosylatedpolypeptidechaincontaining212aminoacids(1-192a.a.)andhavingamolecularmassof23.8kDa(MolecularweightonSDS-PAGEwillappearhigher).ARL14isfusedtoa20aminoacidHis-tagatN-terminus&purifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Formulation
ARL14proteinsolution(0.25mg/ml)containing20mMTris-HClbuffer(pH8.0),2mMDTT,40%glycerol,200mMNaCland1mMEDTA.
StABIlity
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.Store,frozenat-20°Cforlongerperiodsoftime.Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).Avoidmultiplefreeze-thawcycles.
Purity
Aminoacidsequence
MGSSHHHHHHSSGLVPRGSHMGSLGSKNPQTKQAQVLLLGLDSAGKSTLLYKLKLAKDITTIPTIGFNVEMIELERNLSLTVWDVGGQEKMRTVWGCYCENTDGLVYVVDSTDKQRLEESQRQFEHILKNEHIKNVPVVLLANKQDMPGALTAEDITRMFKVKKLCSDRNWYVQPCCALTGEGLAQGFRKLTGFVKSHMKSRGDTLAFFKQN.
SafetyDataSheet
SDS