Cataloguenumber
SARS-003
Introduction
HumancoronavirusNL63alsoknownasHCoV-NL63isatypeofcoronavirusthatwasidentifiedin2004.Amongtheassociateddiseasesaremildtomoderateupperrespiratorytractinfections,bronchiolitis,severelowerrespiratorytractinfectionandcroup.CoronavirusNL63appearsmainlyinyoungchildren,theelderlyandimmunocompromisedpatientswithacuterespiratoryillness.CoronavirusNL63hasaseasonalassociationintemperateclimates.CoronavirusNL63isnotanemergingvirus,butratheronethatcontinuallycirculatesthehumanpopulation.TransmissionofHCoV-NL63islikelythroughdropletexpulsionfromtherespiratorytract,whichmaybecottageswhenclosepersonalcontactoccurs.CoronavirusNL63isabletoendureforupto7daysinrespiratorysecretionsandremainsinfectiveatroomtemperature.Oncethevirushasenteredthehostitbindstocellularreceptorsusingspikeproteins,similartothosefoundinHIV-1.CoronavirusNL63isabletouseAngiotensin-convertingenzyme2(ACE2)asanentryreceptortotargetcells.SincecoronavirusNL63isapositivesingle-strandedRNAvirus,theprocessesofreplicationviatranscriptionandtranslationcanbecarriedoutinthecytoplasmoftheinfectedcell.
Description
RecombinantCoronavirusNL63c-terminalnucleoproteinproducedinE.colicontains130a.a.andpurifiedbyaproprietarychromatographictechnique.
Source
PhysicalAppearance
Formulation
CoronavirusNL63proteinsolutionissuppliedinPBS.
StABIlity
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.Store,frozenat-20°Cforlongerperiodsoftime.Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).Avoidmultiplefreeze-thawcycles.
Purity
Proteinis>95%pureasdeterminedby10%PAGE(coomassiestaining).
SafetyDataSheet
SDS