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Prospec/Norovirus Group-2 P-结构域/Norovirus Group-2衣壳P-结构域重组/100µg/NRV-216-100µg-100µg
Cataloguenumber
NRV-216
Introduction
Humannorovirusisclassifiedintotwogroups,group1&group2.Norwalkvirusisthespecieswhichbelongstogroup1andwasdiscoveredin1968atOhio.Norovirusisafamiliarviruswhichcauseshumangastroenteritiswiththefollowingsymptomsvomiting,diarrheaandsickness.CDCreportrevealedthatthereare19-21millionAmericansinfectedbyNororvirusannuallywith800deaths,1in15peoplewithinfection.Aroundtheworld,thisvirusaffectsabout267millionpeopleandcausesover200,000deathseachyear;theselossesaremostlyinlessdevelopedcountriesandintheveryyoung,elderlyandimmuno-suppressedpopulation,though,mostcasesareself-limitedwithafullrecoverywithinjustafewdays.Norovirusisextremelycontagiousandcanspreadfromhumantohumanthroughinfectedfood,waterorcontaminatedsurfaces.TheoutbreaksusuallyoccurfromNovember-April,whilethepeakisinJanuary.NorovirusisapositivesenseRNAviruswith7.5kbnucleotides,encodingamajorstructuralproteinVP1with50-55kDa.ThefulllengthofVP1capsidcomprisestheinternalN-terminal,Hinge,shell(S)andprotruding(P)domains.Pdomainfrom225to520formsP1-P2-P1structure.Moreover,Pdomainhasareceptorbindingregionwhichrecognizeshumanhisto-bloodgroupantigens(HBGAs).PdomainexpressedinbacteriacanspontaneouslyformaPdimeaswellasaPparticleaggregatedby12Pdimmers.PparticledisplaysanincreasedbindingactivitytoHBGAshigherthanvirus-likeparticle(VLP)formedbythefull-lengthcapsid.Fornorovirusvaccinedevelopment,weconsiderPdomainasagoodcandidate.
Description
TheRecombinantNorovirusGroup-2CapsidP-Domain,E.Coliderived,VA387Straincontainsa.a.from225-520havingaMwof30kDa.Theproteinisfusedtoa6xHistagatN-terminalandpurifiedbychromatographytechniques.
P-domain(225-520a.a.)formsP1-P2-P1structure.P-domainhasareceptorbindingregiontorecognizehumanhisto-bloodgroupantigens(HBGAs).P-domainexpressedin
bacteriacanspontaneouslyformaPdimeandaPparticleaggregatedby12Pdimmers.P-particledisplaysanenhancedbindingactivitytoHBGAshigherthanvirus-likeparticle
(VLP)formedbythefulllengthcapsid.
Source
PhysicalAppearance
Formulation
Phosphatebufferand17mMK2CO3.
Purity
StABIlity
TheRecombinantNorovirusGroup-2P-Domainalthoughstableat4°Cfor1week,shouldbestoredbelow-18°C.
Pleasepreventfreezethawcycles.
SafetyDataSheet
SDS
