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Prospec/H3N2 Canine /Hemagglutinin-Influenza A Virus H3N2 Canine Recombinant/10µg/IHA-050-10µg-10µg

Cataloguenumber
IHA-050
Synonyms
Hemagglutinin
Introduction
H3N2isasubtypeoftheinfluenzaAvirus.Itsnamederivesfromtheformsofthetwokindsofproteinsonthesurfaceofitscoat,hemagglutinin(H)andneuraminidase(N).H3N2exchangesgenesforinternalproteinswithotherinfluenzasubtypes.H3N2hastendedtodominateinprevalenceoverH1N1,H1N2,andinfluenzaB.H3N2straindescendedfromH2N2byantigenicshift,inwhichgenesfrommultiplesubtypesre-assortedtoformanewvirus.BoththeH2N2andH3N2strainscontainedgenesfromavianinfluenzaviruses.H3N2virusesareabletoinfectmammalsandbirds.Inpigs,humans,andbirds,thevirushasmutatedintomanystrains.Hemagglutinin(HA)bindstosialicacid-containingreceptorsonthecellsurface,generatingtheattachmentofthevirusparticletothecell.HAhasavitalpartinthedeterminationofhostrangerestrictionandvirulenceandisinchargeofthediffusionofthevirusintothecellcytoplasmbyfacilitatingthefusionofthemembraneoftheendocytos
Description
CanineH3N2producedinSf9Baculoviruscellsisasingle,glycosylatedpolypeptidechaincontaining336aminoacids(18-344a.a.)andhavingamolecularmassof36.9kDa.(MolecularsizeonSDS-PAGEwillappearatapproximately40-57kDa).
H3N2isexpressedwitha6aminoacidHistagatC-Terminusandpurifiedbyproprietarychromatographictechniques.
Source
Sf9,Baculoviruscells.
PhysicalAppearance
Formulation
CanineH3N2proteinsolution(0.5mg/ml)contains10%glycerol&PhosphateBufferedSaline(pH7.4).
StABIlity
Purity
Aminoacidsequence
ADPNLPGNENNAATLCLGHHAVPNGTIVKTITDDQIEVTNATELVQNSSTGKICNNPHKILDGRDCTLIDALLGDPHCDVFQNETWDLFVERSNAFSNCYPYDVPDYASLRSIVASSGTLEFITEGFTWAGVTQNGGSGACKRGPANGFFSRLNWLTKSGNTYPVLNVTMPNNNNFDKLYIWGVHHPSTNQEQTSLYIQASGRVTVSTRRSQQTIIPNIGSRPLVRGQSGRISVYWTIVKPGDVLVINSNGNLIAPRGYFKMRIGKSSIMRSDAPIDTCISECITPNGSIPNEKPFQNVNKITYGACPKYVKQNTLKLATGMRNVPEKQTHHHHHH.
SafetyDataSheet
SDS