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Prospec/CagA Pylori/Helicobacter Pylori Cytotoxin-Associated Gene A Recombinant/500µg/PRO-2494-500µg-500µg
Cataloguenumber
PRO-2494
Introduction
HelicobacterpyloriisaGramnegativebacteriawhichcolonizesinthehumanstomachandstimulatesgastricmucosalinflammationsleADIngtoacutegastritis-associateddiseasesamongthemareatrophicgastritis,pepticulcerandgastriccancer.
H.pyloriisfoundinhalfoftheworld’spopulationandisdefinedasagroupIcarcinogens..
AnimportantvirulencefactorofH.pyloriisCytotoxin-associatedgeneA(CagA)whichislocatedattheendofanapproximately40-kbclusterofgenescalledcagpathoge¬nicityisland(PAI).
CagPAIencodesatype-IVsecretionsystemandtransfersCagAproteinintotheintracellularregionofgastricepithelialcell.
Thisrelocationinducescellularhyperproliferation,apoptosisandleadingtofailureofgastricepithelialcellinmaintainingitsnormalcytoskeletalstructure.FurThermoreitincreasesthesecretionofcytokinessuchastumornecrosisfactor(TNF)andinterleukin(IL)1,6and8bytheepithelialcells.
TheIL-8leadstoextremeinflammatoryresponsesandalsostimulatestheproductionofoxygenfreeradicalsthatcouldcauseDNAdamagetoadjacentcellsandleadtogeneticmodificationsofgastricepithelialcellswhicharecarcinogenic.
Description
CagAPylorirecombinantantigenisproducedinE.coliexpressingtheH.pyloriCytotoxin-AssociatedGeneAhavingtheMwof34kDa.ADKisfusedtoaHis-tagatC-terminus&purifiedbyproprietarychromatographictechniques.
PurificationMethod
Purifiedbyproprietarychromatographictechnique.
Source
EscherichiaColi.
PhysicalAppearance
Formulation
CagAPylorirecombinantproteinisformulatedin1xPBSpH7.4.
StABIlity
CagAPylorialthoughstableat4°Cfor1week,shouldbestoredbelow-18°C.
Pleasepreventfreezethawcycles.
Purity
Greaterthan95%pureasdeterminedby12%PAGE(Coomassiestaining).
SafetyDataSheet
SDS