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Prospec/Dengue p42/Dengue p42 Recombinant/500µg/DEN-038-500µg-500µg
Cataloguenumber
DEN-038
Introduction
Description
Source
Purity
Formulation
StABIlity
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.Store,frozenat-20°Cforlongerperiodsoftime.Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).Avoidmultiplefreeze-thawcycles.
SafetyDataSheet
SDS
Preparationprotocol
ProtocoltopreparedengueIgG/IgMrapidtest:1.Prepare532to534nmcolloidgold,conjugationwithin24hoursaftercolloidgoldpreparation;2.AdjustcolloidgoldODat1.3to1.4,conjugationconcentrationandpHare16to18ug/mlgoldand7.2;3.Shankingconjugationfor6-8hours,thencentrifugetheconjugationat10,000rpmat18oCfor20min;4.RemovethesupernatnantasmoreaspossIBLe,addthesuppliedgoldsUSPensionbuffer;5.Addthesuppliedblockingreagenttothefinalconcentrationto0.4%;6.Blockingfor1hours,thenaddsucrosetothefinalconcentrationto20%;7.Dippingtheconjugatedgoldtothegoldpad(donotusesodiumphosphateforgoldpadtreatment);8.Afterdrying,assemblegoldpad,thesuppliedsamplepadandabsorbentpadoverbackingcard;9.Add10ulserum/plasmaandaddtwodropsrunningbufferforassay.