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Prospec/Dengue p42/Dengue p42 Recombinant/100µg/DEN-038-100µg-100µg
Catalogue number
DEN-038
Introduction
Description
Source
Purity
Formulation
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Safety Data Sheet
SDS
Preparation protocol
Protocol to prepare dengue IgG/IgM rapid test:1. Prepare 532 to 534nm colloid gold, conjugation within 24 hours after colloid gold preparation;2. Adjust colloid gold OD at 1.3 to 1.4, conjugation concentration and pH are 16 to 18 ug/ml gold and 7.2;3. Shanking conjugation for 6-8 hours, then centrifuge the conjugation at 10,000 rpm at 18oC for 20 min;4. Remove the supernatnant as more as possible, add thesupplied gold suspension buffer;5. Add thesupplied blocking reagentto the final concentration to 0.4%;6. Blocking for 1 hours , then add sucrose to the final concentration to 20%;7. Dipping the conjugated gold to the gold pad ( do not use sodium phosphate for gold pad treatment);8. After drying, assemble gold pad, thesupplied sample padand absorbent pad over backing card;9. Add 10ul serum/plasma and add two drops running buffer for assay.
