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Prospec/Dengue p18/Dengue p18 Recombinant/500µg/DEN-037-500µg-500µg
Catalogue number
DEN-037
Introduction
Description
Source
Purity
Protein is >95% pure as determined by 10% PAGE (coomassie staining).
Formulation
Phosphate buffered saline, 0.02% sodium nitrate and 25mM K2CO3.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Safety Data Sheet
SDS
Preparation protocol
Following is the protocol needed to develop the rapid test by this antigen:1. Prepare λ522 to 525 fresh gold particle within 1-2 days, adjust OD to 1.2 to 1.4.2. conjugate at pH 7.2-7.5 at a concentration of 16ug/ml; 3. conjugate for 2-4 hours, then add 25mM phosphate buffer with 0.2%PVP 10,000 pH8.0 to suspend the conjugate gold pellet.4. Block the suspension with 10% BSA to final 1% BSA.5. Use the sample pad treated with 50mM borate, 1% Tween-20 and 0.5% PVP10,000, pH9.2.6. Prepare the sample buffer with 50-100 mM carbonate buffer with0.5-1.0% Tween-20. The rapid test prepared by this antigen has over 90% sensitivity and specificity over 90% to detect dengue IgM and IgG.
