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Prospec/Chikungunya E1/Chikungunya Wild Type E1 Recombinant/2µg/CHI-001-2µg-2µg
Cataloguenumber
CHI-001
Introduction
ChikungunyaisaninfectioncausedbythechikungunyaviruswhichispassedtohumansbytwospeciesofmosquitoofthegenusAedes:A.albopictusandA.aegypti.Animalreservoirsofthevirusincludemonkeys,birds,cattle,androdents.Thefeaturesofthediseaseareasuddenonsetoffever2-4daysafterexposure.Thefevertypicallylasts2-7days,whiletheassociatedjointpainsusuallylastweeksormonthsbutsometimesyears.Themortalityrateisalittlelessthan1in1,000.ThediseasehasoccurredinoutbreaksinAsia,EuropeandtheAmericassince2004.CHIKVisasingle-strandedpositive-senseRNAgenome,11,800ntslongwhichencodes2openreADIngframes.Thenucleocapsidistightlyenvelopedbyahost-derivedlipidbilayer(envelope)supportingthevirus-encodedenvelopeproteins.80glycoproteinspikesareC-terminallyanchoredwithintheviralenvelope.ThestructuralpolyproteinistranslatedfromaviralsubgenomicmRNA,whileasthe5structuralproteins(capsid,E3,E2,6K,E1)aretranslatedasasinglepolyprotein,fromwhichcapsid(C)iscleavedofftoencapsidate.TheenvelopepolyproteinprecursorE3-E2-6K-E1istranslocatedtotheendoplasmaticreticulum.Polyproteinisprocessedbyhostsignalases,resultinginE3,E2&E1formingviralhetero-trimericspikes.TheviralspikesmajorlycontainsE2andE1facilitatecellreceptorrecognition,cellentrythrupH-dependentendocytosisandsupportviralbudding.
Description
Source
PhysicalAppearance
Formulation
CHIKVE1proteinsolutionin1xD-PBS,pH7.4,0.1%Thimerosal,5mMEDTA,1µg/mlofLeupeptin,andPepstatinA.
StABIlity
Purity
Proteinis95%pureasdeterminedby12.5%SDS-PAGE.
SafetyDataSheet
SDS