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Prospec/Borrelia VisE1/Borrelia Burgdorferi VlsE1 Recombinant/20µg/BOR-024-20µg-20µg
Cataloguenumber
BOR-024
Introduction
BorreliaisapartofthegenusofbacteriaofthespiRochetephylum.Borreliacausesborreliosis,whichisazoonotic,vector-bornediseasetransmittedmainlybyticksandsomebylice,dependingonthespecies.Ofthe36knownspeciesofBorrelia,12aredistinguishedtocauseLymediseaseorborreliosisandaretransmittedbyticks.ThemainBorreliaspeciescausingLymediseaseareBorreliaburgdorferi,Borreliaafzelii,andBorreliagarinii.VariablemajorproteinlikesequenceE1protein(VlsE1)isaborrelialsurfaceproteinwhichisthemostsensitiveproteinforIgGantibodydetectioninallstagesofLymedisease.
Description
RecombinantBorreliaVisE1(variablemajorproteinlikesequenceE1)producedinE.coliisanon-glycosylated,polypeptidechainhavingacalculatedmolecularmassof43kDa.
BorreliaVisE1isexpressedwitha-10xHistagatN-terminusandpurifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Formulation
BorreliaVisE1issuppliedin20mMHEPESbufferpH-8.0,200mMNaCland20%glycerol.
StABIlity
Purity
Greaterthan95.0%asdeterminedbySDS-PAGE.
Applications
WesternblotwithPatientsample.
Immunologicalfunctions
1.BindsIgG-andIgM-typehumanantibodies.2.ImmunodottestwithLymediseasepositive/negativesamples.
SafetyDataSheet
SDS