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Prospec/TPSAB1 Human, Sf9/Tryptase Alpha/Beta 1 Human Recombinant, Sf9/1mg/ENZ-1062-1mg-1mg

Catalogue number
ENZ-1062
Synonyms
Tryptase alpha/beta-1, TPSAB1, TPS1, TPS2, TPSB1, Tryptase I, Tryptase alpha-1.
Introduction
Tryptase alpha/beta-1 (TPSAB1) is a tryptase which is the key neutral protease present in mast cells and is discharged upon the coupled activation-degranulation response of this cell type. TPSAB1 is enzymatically active only as a heparin-stabilized tetramer, and is resistant to all known endogenous proteinase inhibitors. TPSAB1 is implicated as a mediator in the pathogenesis of asthma and other allergic and inflammatory disorders.
Description
TPSAB1 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain (31-275 a.a.) and fused to a 6 aa His Tag at C-terminus containing a total of 251 amino acids and having a molecular mass of 28.2kDa.TPSAB1 shows multiple bands between 28-40kDa on SDS-PAGE, reducing conditions and purified by proprietary chromatographic techniques.
Source
Physical Appearance
Formulation
TPSAB1 protein solution (0.5mg/ml) contains Phosphate Buffered Saline (pH 7.4) & 20% glycerol.
Stability
Purity
Greater than 90.0% as determined by SDS-PAGE.
Amino acid sequence
IVGGQEAPRS KWPWQVSLRV HGPYWMHFCG GSLIHPQWVL TAAHCVGPDV KDLAALRVQL REQHLYYQDQ LLPVSRIIVH PQFYTAQIGA DIALLELEEP VNVSSHVHTV TLPPASETFP PGMPCWVTGW GDVDNDERLP PPFPLKQVKV PIMENHICDA KYHLGAYTGD DVRIVRDDML CAGNTRRDSC QGDSGGPLVC KVNGTWLQAG VVSWGEGCAQ PNRPGIYTRV TYYLDWIHHY VPKKPHHHHH H.
Safety Data Sheet
SDS