Cataloguenumber
PKA-243
Synonyms
KDRD1-7,sKDRD1-7,Kinaseinsertdomainreceptor,Protein-tyrosinekinasereceptorFlk-1,CD309,typeIIIreceptortyrosinekinase,FLK1,VEGFR-2.
Introduction
Endothelialcellsexpressthreedifferentvascularendothelialgrowthfactor(VEGF)receptors,belongingtothefamilyofreceptortyrosinekinases(RTKs).TheyarenamedVEGFR-1(Flt-1),VEGFR-2(KDR/Flk-1),VEGFR-3(Flt-4).Theirexpressionisalmostexclusivelyrestrictedtoendothelialcells,butVEGFR-1canalsobefoundonmonocytes.AllVEGF-receptorshavesevenimmunoglobulin-likeextracellulardomains,asingletransmembraneregionandanintracellularsplittyrosinekinasedomain.VEGFR-2hasaloweraffinityforVEGFthantheFlt-1receptor,butahighersignalingactivity.MitogenicactivityinendothelialcellsismainlymediatedbyVEGFR-2le
ADIngtotheirproliferation.Differentialsplicingoftheflt-1geneleadstotheformationofasecreted,solublevariantofVEGFR-1(sVEGFR-1).Nonaturallyoccuring,secretedformsofVEGFR-2havesofarbeenreported.ThebindingofVEGF165toVEGFR-2isdependentonheparin.
Description
SolubleVEGFR2FcHumanRecombinantfusedwiththeFcpartofhumanIgG1producedinbaculovirusisadisulfide-linkedhomodimeric,glycosylated,polypeptidecontaining968aminoacidsandhavingamolecularmassof145kDa.Thesolublereceptorproteincontainsonlythefirst7extracellulardomains,whichcontainalltheinformationnecessaryforligandbinding.
ThesKDRFcChimeraispurifiedbyproprietarychromatographictechniques.
Source
InsectCells.
PhysicalAppearance
SterileFilteredWhitelyophilized(freeze-dried)powder.
Formulation
KDRfusionproteinwaslyophilizedfromaconcentrated(1mg/ml)sterilesolutioncontaining1xPBSpH-7.2.
Solubility
ItisrecommendedtoreconstitutethelyophilizedVEGFR2insterilewaternotlessthan50µg/ml,whichcanthenbefurtherdilutedtootheraqueoussolutions.
LyophilizedVEGFR-2Fc/Chimeraproteinalthoughstableatroomtemperaturefor3weeks,shouldbestoreddesiccatedbelow-18°C.UponreconstitutionFLK1shouldbestoredat4°Cbetween2-7daysandforfutureusebelow-18°C.Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).Pleasepreventfreeze-thawcycles.
Purity
Greaterthan90.0%asdeterminedbySDS-PAGE.
TheactivityofsVEGFR2/FcwasdeterminedbyitsabilitytoinhibittheVEGF-dependentproliferationofhumanumbilicalveinendothelialcells.
Aminoacidsequence
ASVGLPSVSLDLPRLSIQKDILTIKANTTLQITCRGQRDLDWLWPNNQSGSEQRVEVTECSDGLFCKTLTIPKVIGNDTGAYKCFYRETDLASVIYVYVQDYRSPFIASVSDQHGVVYITENKNKTVVIPCLGSISNLNVSLCARYPEKRFVPDGNRISWDSKKGFTIPSYMISYAGMVFCEAKINDESYQSIMYIVVVVGYRIYDVVLSPSHGIELSVGEKLVLNCTARTELNVGIDFNWEYPSSKHQHKKLVNRDLKTQSGSEMKKFLSTLTIDGVTRSDQGLYTCAASSGLMTKKNSTFVRVHEKPFVAFGSGMESLVEATVGERVRIPAKYLGYPPPEIKWYKNGIPLESNHTIKAGHVLTIMEVSERDTGNYTVILTNPISKEKQSHVVSLVVYVPPQIGEKSLISPVDSYQYGTTQTLTCTVYAIPPPHHIHWYWQLEEECANEPSQAVSVTNPYPCEEWRSVEDFQGGNKIEVNKNQFALIEGKNKTVSTLVIQAANVSALYKCEAVNKVGRGERVISFHVTRGPEITLQPDMQPTEQESVSLWCTADRSTFENLTWYKLGPQPLPIHVGELPTPVCKNLDTLWKLNATMFSNSTNDILIMELKNASLQDQGDYVCLAQDRKTKKRHCVVRQLTVLERVAPTITGNLENQTTSIGESIEVSCTASGNPPPQIMWFKDNETLVEDSGIVLKDGNRNLTIRRVRKEDEGLYTCQACSVLGCAKVEAFFIIEGANASDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSREEMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPMLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK.
SafetyDataSheet
Usage
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.