商品介绍
Catalogue number
ENZ-117
Synonyms
Probable ubiquitin-conjugating enzyme E2 W, Ubiquitin carrier protein W, Ubiquitin-protein ligase W, UBE2W, hUBC-16, FLJ11011.
Introduction
UBE2W receives ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro, UBE2W catalyzes monoubiquitination and "Lys-11"-linked polyubiquitination. UBE2W is broadly expressed, particularly at highest levels in the testis.
Description
UBE2W produced in E.Coli is a single, non-glycosylated polypeptide chain containing 171 amino acids (1-151 a.a.) and having a molecular mass of 19.5kDa.UBE2W is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile filtered colorless solution.
Formulation
UBE2W solution (1mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 10% glycerol, 2mM DTT and 0.1M NaCl.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MASMQKRLQK ELLALQNDPP PGMTLNEKSV QNSITQWIVD MEGAPGTLYE GEKFQLLFKF SSRYPFDSPQ VMFTGENIPV HPHVYSNGHI CLSILTEDWS PALSVQSVCL SIISMLSSCK EKRRPPDNSF YVRTCNKNPK KTKWWYHDDT C.
Stability
UBE2W Human Recombinant although stable at 4°C for 1 week, should be stored below -18°C. Please prevent freeze thaw cycles.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Safety Data Sheet
Usage
ProSpec"s products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
品牌介绍
ProSpec-Tany TechnoGene Ltd.公司创立人Dr. Tanchum (Tany) Amarant 主要致力于蛋白生物化学方面的研究工作,1947年出生于以色列。1991-1997创建ReProGen 公司,从事细胞因子和趋化因子的大量生产及纯化;1997-2000创建ProSpec-TechnoGene,主要生产用于生命科学研究的各种重组蛋白及相关抗体。拥有专利United States Patent 5434135。
ProSpec is an evolving biotech company providing highly purified proteins worldwide which serve the research community with cytokine-related products for cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cell research.
Among our proteins are Interferons, Interleukins, Bone Morphogenic Proteins, Tumor Necrosis factors, Leptins, Stem Cell factors, Prolactins, Chemokines, Antibodies, Peptides, Enzymes, Viral Antigens, various growth factors such as: Platelet Derived, Epidermal, Insulin Like, Nerve, Connective Tissue, Vascular Endothelial, Fibroblast and more.....
Our research team and scientists have been able to assemble more than 1700 recombinant proteins, peptides and antibodies for your research needs. The proteins are rigorously tested to meet the research and development demand for excellent quality, uncompromising biological activity at competitive prices.
ProSpec has established sterling reputation among the scientific community, a reputation built on innovative products, customer service and attention to detail. Our reputation for quality is an essential factor in achieving a favorable position in the market place and the benefits associated with manufacturing top quality products.