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Prospec/KMT5A Human/Lysine Methyltransferase 5A Human Recombinant/1mg/ENZ-1080-1mg-1mg
Cataloguenumber
ENZ-1080
Synonyms
KMT5A,PR-Set7,SET07,SET8,SETD8,H4-K20-HMTaseKMT5A.
Introduction
LysineMethyltransferase5A(KMT5A)isanenzymewhichcatalyzesbothhistonesandnon-histoneproteins.KMT5Acontributestothemaintenanceofproperhigher-orderstructureofDNAduringmitosis.KMT5Atakespartincell-cycle-dependenttranscriptionalsilencingandmitoticregulationinmetazoans.KMT5Aplaysaroleasabarriertopreventcellularsenescencethroughchromatinmediatedregulationofsenescence-associatedmetabolicremodeling.KMT5Amediatesmonomethylationofp53/TP53at"Lys-382",whichleadstorepressp53/TP53-targetgenes.ThelossofKMT5Asimultaneouslystimulatenucleolarfunctionandretinoblastomaprotein-mediatedmitochondrialmetabolism.
Description
KMT5AproducedinSf9Baculoviruscellsisasingle,glycosylatedpolypeptidechaincontaining165aminoacids(195-352a.a.)andhavingamolecularmassof18.9kDa(Migratesat18-28kDaonSDS-PAGEunderreducingconditions).KMT5Aisexpressedwitha7aminoacidHistagatC-Terminusandpurifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Formulation
KMT5Aproteinsolution(0.25mg/ml)contains20mMTris-HClbuffer(pH8.0),5mMDTT,0.2MNaCl,1mMEDTAand50%glycerol.
StABIlity
Purity
Greaterthan85.0%asdeterminedbySDS-PAGE.
Aminoacidsequence
MKAELQSEERKRIDELIESGKEEGMKIDLIDGKGRGVIATKQFSRGDFVVEYHGDLIEITDAKKREALYAQDPSTGCYMYYFQYLSKTYCVDATRETNRLGRLINHSKCGNCQTKLHDIDGVPHLILIASRDIAAGEELLYDYGDRSKASIEAHPWLKHHHHHHH.
SafetyDataSheet
SDS