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Prospec/HNMT Human, Active/Histamine N-Methyltransferase Human Recombinant, Active/5µg/ENZ-1071-5µg-5µg
Cataloguenumber
ENZ-1071
Synonyms
HMT,HNMT-S1,HNMT-S2,MRT51.
Introduction
HistamineN-MethyltransferaseorHNMT,islocatedinthecellcytosol.Sadenosyl-L-methionineactsasamethyldonorforHNMTwhichinactivateshistamine.Byinactivationofhistamine,itaffectstheimmunesystem’sresponse.HNMTactsonhistamineinbodytissuessuchaskidney,centralnervoussystemandbronchus.Theproteinhasacrucialpartintheairwayresponsetohistamine&histaminedegradationprocessandregulation.
Description
HNMTHumanRecombinantproducedinE.Coliisasingle,non-glycosylatedpolypeptidechaincontaining328aminoacids(1-292a.a)andhavingamolecularmassof37.4kDa.HNMTisfusedtoa36aminoacidHis-tagatN-terminus&purifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Formulation
HNMTproteinsolution(1mg/ml)containing20mM,Tris-Hcl(pH8.0)and10%glycerol.
StABIlity
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.Store,frozenat-20°Cforlongerperiodsoftime.Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).Pleaseavoidfreeze-thawcycles.
Aminoacidsequence
MRGSHHHHHHGMASMTGGQQMGRDLYDDDDKDRWGSMASSMRSLFSDHGKYVESFRRFLNHSTEHQCMQEFMDKKLPGIIGRIGDTKSEIKILSIGGGAGEIDLQILSKVQAQYPGVCINNEVVEPSAEQIAKYKELVAKTSNLENVKFAWHKETSSEYQSRMLEKKELQKWDFIHMIQMLYYVKDIPATLKFFHSLLGTNAKMLIIVVSGSSGWDKLWKKYGSRFPQDDLCQYITSDDLTQMLDNLGLKYECYDLLSTMDISDCFIDGDENGDLLWDFLTETCNFNATAPPDLRAELGKDLQEPEFSAKKEGKVLFNNTLSFIVIEA.
BIOLOGicalActivity
Specificactivityis>200nmol/min/mg,andisdefinedastheamountofenzymethattransfer1.0nmoleofmethylgroupperminuteat37°C.
SafetyDataSheet
SDS
Purity
Greaterthan90.0%asdeterminedbySDS-PAGE.