GlutathioneS-transferaseP,GSTclass-pi,GSTP1-1,GSTP1,FAEES3,GST3,PI,DFN7.
GSTP1isapolymorphicgeneencodingactive,functionallydifferentGSTP1variantproteinsthatarebelievedtofunctioninxenobioticmetabolismandhaveapartinsusceptibilitytocancer,andotherdiseases.GSTP1isaglutathioneS-transferasebelongingtothepiclass.GSTfamilyenzymesplayasignificantroleindetoxificationbycatalyzingtheconjugationofmanyhydrophobicandelectrophiliccompoundswithreducedglutathione.Baseduponthebiochemical,immunologicandstructuralpropertiesofthesolubleGSTstheyaregroupedinto4mainclasses:alpha,mu,pi,andtheta.TheGSTP1enzymeactsbycatalyzingthereactionofglutathionewithanacceptormoleculetoformaSulfur-substitutedglutathione.Thereactionsemployingglutathionecontributethetransformationofabroadrangeofelectrophiles,includingreactiveproductsoflipid,protein,carcinogens,therapeuticdrugs,environmentaltoxins,andproductsofoxidativestress.TheGSTP1inactivationthroughCpGhypermethylationisfrequentinpituitaryadenomasandmaybeafactorinaggressivepituitarytumorbehavior.GSTP1ismaybeatranscriptionaltargetofthep53tumorsuppressorgene.Single-nucleotidepolymorphisminGSTP1islinkedtomodifiedproteinbinding,whichinfluenceGSTP1"scontributiontocarcinogenanddrugmetabolism,andposs
IBLydiseasepathogenesisand/ordrugresponse.GST-pimighthavecentralrolesinproliferationofandrogen-independenthumanprostatecancercells.
GSTP1HumanRecombinantfusedwith36aminoacidHistagatN-terminusproducedinE.Coliisasingle,non-glycosylated,polypeptidechaincontaining246aminoacids
(1-210a.a.)Andhavingamolecularmassof27.4kDa.
TheGSTP1ispurifiedbyproprietarychromatographictechniques.
EscherichiaColi.
SterileFilteredcolorlesssolution.
TheGSTP1solution(0.5mg/ml)contains20mMTris-HClbuffer(pH7.0),30%glycerol,1mMEDTAand0.1mMPMSF.
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.
Store,frozenat-20°Cforlongerperiodsoftime.Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).Avoidmultiplefreeze-thawcycles.
Greaterthan95.0%asdeterminedbySDS-PAGE.
Specificactivityis>80units/mg,andisdefinedastheamountofenzymethatconjugate1.0umoleof1-chloro-2,4-dinitrobenzene(CDNB)withreducedglutathioneperminuteatpH6.5at25°C.
MRGSHHHHHHGMASMTGGQQMGRDLYDDDDKDRWGSMPPYTVVYFPVRGRCAALRMLLADQGQSWKEEVVTVETWQEGSLKASCLYGQLPKFQDGDLTLYQSNTILRHLGRTLGLYGKDQQEAALVDMVNDGVEDLRCKYISLIYTNYEAGKDDYVKALPGQLKPFETLLSQNQGGKTFIVGDQISFADYNLLDLLLIHEVLAPGCLDAFPLLSAYVGRLSARPKLKAFLASPEYVNLPINGNGKQ.
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.