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Prospec/GST, 218 a.a./Glutathione S-Transferase, 218 a.a. Recombinant/2µg/ENZ-1079-2µg-2µg
Cataloguenumber
ENZ-1079
Synonyms
GlutathioneS-Transferase,GST,GlutathioneS-transferaseclass-mu28kDaisozyme,GST28,EC2.5.1.18,Sj28GST,Sj28antigen,Sj26antigen.
Introduction
AntioxidantenzymeGlutathioneS-Transferase(GST)isthoughttodotheprimarycellulardefensemechanismagainstreactiveoxygenspecies.GSTreduceslipidhydroperoxidesthroughitsSe-independentglutathioneperoxidaseactivity.Theenzymealsodetoxifieslipidperoxidationendproductssuchas4-hydroxynonenal(4-HNE).ThesolubleGSTisa26kDaproteinwhichoccursasadimerinallaerobicorganisms.Eachmonomerhastwodomains,onethatbindsGSHandisan/-structuresimilartothioredoxinandtheother,allhelical,thatbindsthehydrophobicsubstrate.TheGST-fusionproteinexpressionsystemisawidelyusedrecombinantproteinexpressionsystemthatallowsapeptideoraregulatoryproteindomaintobeexpressedasafusiontotheC-terminusofSchistosomajaponicumGST.FusionproteinsalsopossessGST-enzymaticactivityandcanundergodimerizationsimilartoinvivo.ThefusionproteincanbepurifiedviaGST-affinitycolumnchromatography.Inmostcases,thedesiredpeptidesordomainsareremovedfromGSTbyapplyingaspecificproteasethatrecognizesandcleavesthelinkerbetweentheproteindomainandGST.Thetechniquehasbeenwidelyusedtogeneratedifferentkindsofproteinsforcrystallization,molecularimmunologystudies,theproductionofvaccinesandstudiesinvolvingprotein-proteinandprotein-DNAinteractions.
Description
GSTRecombinantproducedinE.Coliisasingle,non-glycosylatedpolypeptidechaincontaining218aminoacids(1-218a.a)andhavingamolecularmassof25.4kDa
Source
EscherichiaColi. |
PhysicalAppearance
Formulation
GSTproteinsolution(1mg/ml)containingPBSand10%glycerol.
StABIlity
Purity
Greaterthan90.0%asdeterminedbySDS-PAGE.
BIOLOGicalActivity
TheSpecificactivityis>30units/mg,andisdefinedastheamountofenzymethatconjugate1.0umoleof1-chloro-2,4-dinitrobenzene(CDNB)withreducedglutathioneperminuteatpH6.5at25C.
SafetyDataSheet
SDS
Aminoacidsequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK.
