>
产品中心 >
Other_enzymes >
Prospec/GALNT1 Human/Polypeptide N-Acetylgalactosaminyltransferase 1 Human Recombinant/2µg/enz-1098-2µg-2µg
Cataloguenumber
enz-1098
Synonyms
PolypeptideN-acetylgalactosaminyltransferase1,GALNT1,GALNAC-T1
Introduction
PolypeptideN-Acetylgalactosaminyltransferase1(Galnt1)isapartoftheUDP-N-acetyl-alpha-D-galactosamine:polypeptideN-acetylgalactosaminyltransferase(GalNAc-T)familyofenzymes.TheinitialreactioninO-linkedoligosaccharidebiosynthesisiscatalyzedbyGlant1,thetransferofanN-acetyl-D-galactosamineresiduetoaserineorthreonineresidueontheproteinreceptor.Moreover,Galnt1isimplicatedintheglycosylationofproteinsvitalforboneformationforinstanceosteopontinandbonesialoprotein.
Description
GALNT1producedinSf9Insectcellsisasingle,glycosylatedpolypeptidechaincontaining528aminoacids(41-559a.a.)andhavingamolecularmassof60.4kDa(MolecularsizeonSDS-PAGEwillappearatapproximately50-70kDa).GALNT1isexpressedwithan9aminoacidHistagatC-Terminusandpurifiedbyproprietarychromatographictechniques.
Source
Sf9,Insectcells.
PhysicalAppearance
Sterilefilteredcolorlesssolution.
Formulation
GALNT1proteinsolution(1mg/ml)contains20mMTris-HClbuffer(pH8.0)containing0.1MNaCland10%glycerol.
StABIlity
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.Store,frozenat-20°Cforlongerperiodsoftime.Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).Avoidmultiplefreeze-thawcycles.
Purity
Greaterthan90.0%asdeterminedbySDS-PAGE.
BIOLOGicalActivity
Thespecificactivitywhichisdefinedastheamountofenzymethattransfer1.0pmoleofGalNAcfromUDP-GalNActopeptideEA2perminuteatpH8.0at37Cis>300pmol/min/ug.
Aminoacidsequence
ADPGLPAGDVLEPVQKPHEGPGEMGKPVVIPKEDQEKMKEMFKINQFNLMASEMIALNRSLPDVRLEGCKTKVYPDNLPTTSVVIVFHNEAWSTLLRTVHSVINRSPRHMIEEIVLVDDASERDFLKRPLESYVKKLKVPVHVIRMEQRSGLIRARLKGAAVSKGQVITFLDAHCECTVGWLEPLLARIKHDRRTVVCPIIDVISDDTFEYMAGSDMTYGGFNWKLNFRWYPVPQREMDRRKGDRTLPVRTPTMAGGLFSIDRDYFQEIGTYDAGMDIWGGENLEISFRIWQCGGTLEIVTCSHVGHVFRKATPYTFPGGTGQIINKNNRRLAEVWMDEFKNFFYIISPGVTKVDYGDISSRVGLRHKLQCKPFSWYLENIYPDSQIPRHYFSLGEIRNVETNQCLDNMARKENEKVGIFNCHGMGGNQVFSYTANKEIRTDDLCLDVSKLNGPVTMLKCHHLKGNQLWEYDPVKLTLQHVNSNQCLDKATEEDSQVPSIRDCNGSRSQQWLLRNVTLPEIFHHHHHH.
SafetyDataSheet
SDS
