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Prospec/TEK Human/TEK Tyrosine Kinase Endothelial Human Recombinant/5µg/PKA-110-5µg-5µg
Cataloguenumber
PKA-110
Synonyms
TEKReceptorTyrosineKinase,TyrosineKinaseWithIgAndEGFHomologyDomains-2,TunicaInternaEndothelialCellKinase,Tyrosine-ProteinKinaseReceptorTIE-2,Tyrosine-ProteinKinaseReceptorTEK,TEKTyrosineKinase,Endothelial,EndothelialTyrosineKinase,EC2.7.10.1,VMCM1,VMCM,TIE2,VenousMalformations,MultipleCutaneousAndMucosal,Angiopoietin-1Receptor,CD202bAntigen,EC2.7.10,P140TEK,CD202B,GLC3E,TIE-2,HTIE2.
Introduction
TIE-1(tyrosinekinasewithIgandEGFhomologydomains1)andTIE-2/Tekcompriseareceptortyrosinekinase(RTK)subfamilywithuniquestructuralcharacteristics:twoimmunoglobulin-likedomainsflankingthreeepidermalgrowthfactor(EGF)-likedomainsandfollowedbythreefibronectintypeIII-likerepeatsintheextracellularregionandasplittyrosinekinasedomaininthecytoplasmicregion.ThesereceptorsareexpressedprimarilyonendothelialandhematopoieticProgenitorcellsandplaycriticalrolesinangiogenesis,vasculogenesisandhematopoiesis.HumanTIE-1CDNAencodesa1122aminoacid(aa)residueprecursorproteinwithan18residueputativesignalpeptide,a726residueextracellulardomainanda353residuecytoplasmicdomain.Twoligands,angiopoietin-1(Ang1)andangiopoietin-2(Ang2),whichbindTIE-2withhigh-affinityhavebeenidentified.Ang2hasbeenreportedtoactasanantagoNISTforAng1.MiceengineeredtooverexpressAng2ortolackAng1orTie-1displaysimilarangiogenicdefects.
Description
TEKproducedinSf9Baculoviruscellsisasingle,glycosylatedpolypeptidechaincontaining965aminoacids(23-748a.a.)andhavingamolecularmassof107.9kDa.(MolecularsizeonSDS-PAGEwillappearatapproximately100-150kDa).TEKisexpressedwitha239aminoacidhIgG-His-tagatC-Terminusandpurifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Formulation
TEKproteinsolution(0.5mg/ml)contains10%glycerol&PhosphateBufferedSaline(pH7.4).
StABIlity
Purity
Greaterthan90.0%asdeterminedbySDS-PAGE.
Aminoacidsequence
AMDLILINSLPLVSDAETSLTCIASGWRPHEPITIGRDFEALMNQHQDPLEVTQDVTREWAKKVVWKREKASKINGAYFCEGRVRGEAIRIRTMKMRQQASFLPATLTMTVDKGDNVNISFKKVLIKEEDAVIYKNGSFIHSVPRHEVPDILEVHLPHAQPQDAGVYSARYIGGNLFTSAFTRLIVRRCEAQKWGPECNHLCTACMNNGVCHEDTGECICPPGFMGRTCEKACELHTFGRTCKERCSGQEGCKSYVFCLPDPYGCSCATGWKGLQCNEACHPGFYGPDCKLRCSCNNGEMCDRFQGCLCSPGWQGLQCEREGIPRMTPKIVDLPDHIEVNSGKFNPICKASGWPLPTNEEMTLVKPDGTVLHPKDFNHTDHFSVAIFTIHRILPPDSGVWVCSVNTVAGMVEKPFNISVKVLPKPLNAPNVIDTGHNFAVINISSEPYFGDGPIKSKKLLYKPVNHYEAWQHIQVTNEIVTLNYLEPRTEYELCVQLVRRGEGGEGHPGPVRRFTTASIGLPPPRGLNLLPKSQTTLNLTWQPIFPSSEDDFYVEVERRSVQKSDQQNIKVPGNLTSVLLNNLHPREQYVVRARVNTKAQGEWSEDLTAWTLSDILPPQPENIKISNITHSSAVISWTILDGYSISSITIRYKVQGKNEDQHVDVKIKNATITQYQLKGLEPETAYQVDIFAENNIGSSNPAFSHELVTLPESQAPADLGGGKMLLLEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGKHHHHHH.
SafetyDataSheet
SDS
