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Prospec/SUMF1 Human, Sf9/Sulfatase Modifying Factor 1 Human Recombinant, Sf9/2µg/PRO-2619-2µg-2µg
Cataloguenumber
PRO-2619
Synonyms
SUMF1,AAPA3037,FGE,UNQ3037,Formylglycine-generatingenzyme,C-alpha-formylglycine-generatingenzyme1,Sulfatase-modifyingfactor1.
Introduction
SUMF1isapartoftheSUMFproteinfamily.SUMF1catalyzesthehydrolysisofsulfateestersbyoxidizingacysteineresidueinthesubstratesulfatasetoanactivesite3-oxoalanineresiduecalledC-alpha-formylglycine.Mutationsinthisgenewillcausemultiplesulfatasedeficiencymeaningalysosomalstoragedisorder.
Description
SUMF1producedinSf9Insectcellsisasingle,glycosylatedpolypeptidechaincontaining347aminoacids(34-374.a.)andhavingamolecularmassof38.1kDa(MolecularsizeonSDS-PAGEwillappearatapproximately40-57kDa).SUMF1isexpressedwitha6aminoacidHistagatC-Terminusandpurifiedbyproprietarychromatographictechniques.
Source
Sf9,Insectcells.
PhysicalAppearance
Formulation
SUMF1proteinsolution(0.25mg/ml)containsPhosphateBufferedSaline(pH7.4)and10%glycerol.
StABIlity
Purity
Greaterthan85.0%asdeterminedbySDS-PAGE.
Aminoacidsequence
SQEAGTGAGAGSLAGSCGCGTPQRPGAHGSSAAAHRYSREANAPGPVPGERQLAHSKMVPIPAGVFTMGTDDPQIKQDGEAPARRVTIDAFYMDAYEVSNTEFEKFVNSTGYLTEAEKFGDSFVFEGMLSEQVKTNIQQAVAAAPWWLPVKGANWRHPEGPDSTILHRPDHPVLHVSWNDAVAYCTWAGKRLPTEAEWEYSCRGGLHNRLFPWGNKLQPKGQHYANIWQGEFPVTNTGEDGFQGTAPVDAFPPNGYGLYNIVGNAWEWTSDWWTVHHSVEETLNPKGPPSGKDRVKKGGSYMCHRSYCYRYRCAARSQNTPDSSASNLGFRCAADRLPTMDHHHHHH.
SafetyDataSheet
SDS