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Prospec/PKM2 Mouse/Tumour Type M2 Pyruvate Kinase Mouse Recombinant/1mg/PKA-097-1mg-1mg
Cataloguenumber
PKA-097
Synonyms
PyruvatekinaseisozymesM1/M2,EC2.7.1.40,Pyruvatekinasemuscleisozyme,Pyruvatekinase2/3,Cytosolicthyroidhormone-bindingprotein,CTHBP,THBP1,M2PK,PKM2,PK3,PK2,PKM,TCB,OIP3,MGC3932,TumorTypeM2PyruvateKinase.
Introduction
Pyruvatekinaseisakeyenzymeintheglycolyticpathway.TheM2isoenzymeofpyruvatekinaseisspecificallyexpressedathighlevelsintumorcells,andcanbemeasuredinplasmaofpatientswithadvancedbreastcancer.TheMarkerisusefulformeasuringdiseaseactivity,sensitivitytochemotherapyandrecurrence.
Description
PKM2MouseRecombinantproducedinE.Coliisasingle,non-glycosylatedpolypeptidechaincontaining554aminoacids(1-531a.a)andhavingamolecularmassof60.2kDa.PKM2isfusedtoa23aminoacidHis-tagatN-terminus&purifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Formulation
PKM2proteinsolution(0.5mg/ml)containing20mMTris-HCl(pH8.5),0.2MNaCl,1mMDTTand30%glycerol.
StABIlity
Purity
Greaterthan85%asdeterminedbySDS-PAGE.
Aminoacidsequence
MGSSHHHHHHSSGLVPRGSHMGSMPKPHSEAGTAFIQTQQLHAAMADTFLEHMCRLDIDSAPITARNTGIICTIGPASRSVEMLKEMIKSGMNVARLNFSHGTHEYHAETIKNVREATESFASDPILYRPVAVALDTKGPEIRTGLIKGSGTAEVELKKGATLKITLDNAYMEKCDENILWLDYKNICKVVEVGSKIYVDDGLISLQVKEKGADFLVTEVENGGSLGSKKGVNLPGAAVDLPAVSEKDIQDLKFGVEQDVDMVFASFIRKAADVHEVRKVLGEKGKNIKIISKIENHEGVRRFDEILEASDGIMVARGDLGIEIPAEKVFLAQKMMIGRCNRAGKPVICATQMLESMIKKPRPTRAEGSDVANAVLDGADCIMLSGETAKGDYPLEAVRMQHLIAREAEAAIYHLQLFEELRRLAPITSDPTEAAAVGAVEASFKCCSGAIIVLTKSGRSAHQVARYRPRAPIIAVTRNPQTARQAHLYRGIFPVLCKDAVLNAWAEDVDLRVNLAMDVGKARGFFKKGDVVIVLTGWRPGSGFTNTMRVVPVP.
BIOLOGicalActivity
Specificactivity:>500units/mg.Oneunitwillconvert1pmoleofphospho(enol)pyruvatetopyruvateperminuteatpH7.5at37°C.
SafetyDataSheet
SDS