>
产品中心 >
Other_enzymes >
Prospec/PKM2 Human/Tumor Type M2 Pyruvate Kinase Human Recombinant/10µg/PKA-339-10µg-10µg

Cataloguenumber
PKA-339
Synonyms
Introduction
Description
PKM2HumanRecombinantfusedwitha20aminoacidHistagatN-terminusproducedinE.Coliisasingle,non-glycosylated,polypeptidechaincontaining551aminoacids(1-531a.a.)andhavingamolecularmassof60.1kDa.ThePKM2ispurifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Formulation
ThePKM2solution(1mg/ml)contains20mMTris-HClbuffer(pH8.0)and10%glycerol.
StABIlity
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.
Store,frozenat-20°Cforlongerperiodsoftime.
Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Avoidmultiplefreeze-thawcycles.
Purity
Greaterthan95.0%asdeterminedbySDS-PAGE.
Aminoacidsequence
MGSSHHHHHHSSGLVPRGSHMSKPHSEAGTAFIQTQQLHAAMADTFLEHMCRLDIDSPPITARNTGIICTIGPASRSVETLKEMIKSGMNVARLNFSHGTHEYHAETIKNVRTATESFASDPILYRPVAVALDTKGPEIRTGLIKGSGTAEVELKKGATLKITLDNAYMEKCDENILWLDYKNICKVVEVGSKIYVDDGLISLQVKQKGADFLVTEVENGGSLGSKKGVNLPGAAVDLPAVSEKDIQDLKFGVEQDVDMVFASFIRKASDVHEVRKVLGEKGKNIKIISKIENHEGVRRFDEILEASDGIMVARGDLGIEIPAEKVFLAQKMMIGRCNRAGKPVICATQMLESMIKKPRPTRAEGSDVANAVLDGADCIMLSGETAKGDYPLEAVRMQHLIAREAEAAIYHLQLFEELRRLAPITSDPTEATAVGAVEASFKCCSGAIIVLTKSGRSAHQVARYRPRAPIIAVTRNPQTARQAHLYRGIFPVLCKDPVQEAWAEDVDLRVNFAMNVGKARGFFKKGDVVIVLTGWRPGSGFTNTMRVVPVP.
BIOLOGicalActivity
TheSpecificactivityis>25,000pmol/min/ug.1unitwillform1pmolofphospho(enol)pyruvatetopyruvateperminuteatpH7.5at37°C.
SafetyDataSheet
SDS